BMRB Entry 11403

Name: Solution structure of Zinc finger domain 7 in Zinc finger protein 32
Published: 2011-09-08

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11403

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of Zinc finger domain 7 in Zinc finger protein 32

Deposition date:

2010-09-09

Original release date:

2011-09-08

Authors:

Tsuda, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.

Citation:

Citation: Tsuda, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution structure of Zinc finger domain 7 in Zinc finger protein 32" .

Assembly members:

Assembly members:
Zinc finger domain, polymer, 42 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P061204-07

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Zinc finger domain: GSSGSSGGETPYLCGQCGKS FTQRGSLAVHQRSCSQSGPS SG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	130
15N chemical shifts	35
1H chemical shifts	197

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Zinc finger domain	1
2	ZINC ION	2

Entities:

Entity 1, Zinc finger domain 42 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLU

THR

2

PRO

TYR

LEU

CYS

GLY

GLN

CYS

GLY

LYS

SER

3

PHE

THR

GLN

ARG

GLY

SER

LEU

ALA

VAL

HIS

4

GLN

ARG

SER

CYS

SER

GLN

SER

GLY

PRO

SER

5

SER

GLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: zinc finger domain mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20060702, Delaglio F. - processing

NMRView v5.0.4, Johnson B.A. - data analysis

Kujira v0.9825, Kobayashi N. - data analysis

CYANA v2.1, Guntert P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz