BMRB Entry 11402

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11402

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of chromo domain 2 in Chromodomain-helicase-DNA-binding protein 6

Deposition date:

2010-09-09

Original release date:

2011-09-08

Authors:

Tsuda, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.

Citation:

Citation: Tsuda, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution structure of chromo domain 2 in Chromodomain-helicase-DNA-binding protein 6" .

Assembly members:

Assembly members:
Chromo domain, UNP residues 371-431, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060904-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Chromo domain, UNP residues 371-431: GSSGSSGNPDYVEVDRILEV AHTKDAETGEEVTHYLVKWC SLPYEESTWELEEDVDPAKV KEFESLQV

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	287
15N chemical shifts	63
1H chemical shifts	441

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Chromo domain, UNP residues 371-431	1

Entities:

Entity 1, Chromo domain, UNP residues 371-431 68 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASN

PRO

ASP

2

TYR

VAL

GLU

VAL

ASP

ARG

ILE

LEU

GLU

VAL

3

ALA

HIS

THR

LYS

ASP

ALA

GLU

THR

GLY

GLU

4

GLU

VAL

THR

HIS

TYR

LEU

VAL

LYS

TRP

CYS

5

SER

LEU

PRO

TYR

GLU

SER

THR

TRP

GLU

6

LEU

GLU

ASP

VAL

ASP

PRO

ALA

LYS

VAL

7

LYS

GLU

PHE

GLU

SER

LEU

GLN

VAL

Samples:

sample_1: Chromo domain, UNP residues 371-431, [U-13C; U-15N], 1.16 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20060702, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9825, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2EPB
DBJ	BAG59500
GB	AAI72397 AAK56405 AAN59903 EAW75974 EAW75975
REF	NP_001230229 NP_115597 XP_001502820 XP_002721122 XP_002806815
SP	Q8TD26
AlphaFold	Q8TD26