BMRB Entry 11399

Name: solution structure of RNA binding domain in PTB-like protein L
Published: 2011-09-08

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11399

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

solution structure of RNA binding domain in PTB-like protein L

Deposition date:

2010-09-09

Original release date:

2011-09-08

Authors:

Tsuda, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.

Citation:

Citation: Tsuda, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "solution structure of RNA binding domain in PTB-like protein L" .

Assembly members:

Assembly members:
RNA recognition motif, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040921-04

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA recognition motif: GSSGSSGDKMDGAPSRVLHI RKLPGEVTETEVIALGLPFG KVTNILMLKGKNQAFLELAT EEAAITMVNYYSAVTPHLRN QPIYIQYSNHKELKTSGPSS G

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	413
15N chemical shifts	87
1H chemical shifts	663

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RNA recognition motif	1

Entities:

Entity 1, RNA recognition motif 101 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASP

LYS

MET

2

ASP

GLY

ALA

PRO

SER

ARG

VAL

LEU

HIS

ILE

3

ARG

LYS

LEU

PRO

GLY

GLU

VAL

THR

GLU

THR

4

GLU

VAL

ILE

ALA

LEU

GLY

LEU

PRO

PHE

GLY

5

LYS

VAL

THR

ASN

ILE

LEU

MET

LEU

LYS

GLY

6

LYS

ASN

GLN

ALA

PHE

LEU

GLU

LEU

ALA

THR

7

GLU

ALA

ILE

THR

MET

VAL

ASN

TYR

8

TYR

SER

ALA

VAL

THR

PRO

HIS

LEU

ARG

ASN

9

GLN

PRO

ILE

TYR

ILE

GLN

TYR

SER

ASN

HIS

10

LYS

GLU

LEU

LYS

THR

SER

GLY

PRO

SER

11

GLY

Samples:

sample_1: RNA recognition motif, [U-13C; U-15N], 1.34 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9297, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2CQ1
DBJ	BAB71742 BAB71743 BAD92503 BAE20599 BAE20681
EMBL	CAB54073
GB	AAF14284 AAF21807 AAH10255 AAH16582 AAH82076
PIR	JC7526
REF	NP_001005555 NP_001103551 NP_001287914 NP_001287915 NP_001287916
SP	Q66H20 Q91Z31 Q9UKA9
TPG	DAA00060 DAA00061 DAA31417
AlphaFold	Q66H20 Q91Z31 Q9UKA9