BMRB Entry 11390

Name: Solution structure of the death domain of Ankyrin-1
Published: 2011-09-08

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11390

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the death domain of Ankyrin-1

Deposition date:

2010-09-08

Original release date:

2011-09-08

Authors:

Tanabe, W.; Suzuki, S.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.

Citation:

Citation: Tanabe, W.; Suzuki, S.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution structure of the death domain of Ankyrin-1" .

Assembly members:

Assembly members:
death domain, UNP residues 1394-1497, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060123-20

Entity Sequences (FASTA):

Entity Sequences (FASTA):
death domain, UNP residues 1394-1497: GSSGSSGPGSLSGTEQAEMK MAVISEHLGLSWAELARELQ FSVEDINRIRVENPNSLLEQ SVALLNLWVIREGQNANMEN LYTALQSIDRGEIVNMLEGS GRQSRNLKPDR

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	409
15N chemical shifts	112
1H chemical shifts	709

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	death domain, UNP residues 1394-1497	1

Entities:

Entity 1, death domain, UNP residues 1394-1497 111 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

GLY

SER

2

LEU

SER

GLY

THR

GLU

GLN

ALA

GLU

MET

LYS

3

MET

ALA

VAL

ILE

SER

GLU

HIS

LEU

GLY

LEU

4

SER

TRP

ALA

GLU

LEU

ALA

ARG

GLU

LEU

GLN

5

PHE

SER

VAL

GLU

ASP

ILE

ASN

ARG

ILE

ARG

6

VAL

GLU

ASN

PRO

ASN

SER

LEU

GLU

GLN

7

SER

VAL

ALA

LEU

ASN

LEU

TRP

VAL

ILE

8

ARG

GLU

GLY

GLN

ASN

ALA

ASN

MET

GLU

ASN

9

LEU

TYR

THR

ALA

LEU

GLN

SER

ILE

ASP

ARG

10

GLY

GLU

ILE

VAL

ASN

MET

LEU

GLU

GLY

SER

11

GLY

ARG

GLN

SER

ARG

ASN

LEU

LYS

PRO

ASP

12

ARG

Samples:

sample_1: death domain, UNP residues 1394-1497 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20060702, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2YQF 2YVI
DBJ	BAD92655 BAG54351
EMBL	CAA34610 CAA34611
GB	AAA51732 AAB47805 AAI56402 EAW63241 EAW63242
PIR	B35049
PRF	1605244A
REF	NP_000028 NP_001135918 NP_065208 NP_065209 NP_065210
SP	P16157
AlphaFold	P16157