BMRB Entry 11375

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PDB ID: 2rne
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11375
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assigned chemical shifts of the second RNA recognition motif (RRM) of TIA-1 with RNA (UUUUU)

Deposition date:

2010-09-08

Original release date:

2010-11-30

Authors:

Takahashi, M.; Kuwasako, K.; Abe, C.; Tsuda, K.; Inoue, M.; Terada, T.; Shirouzu, M.; Kobayashi, N.; Kigawa, T.; Taguchi, S.; Guntert, P.; Hayashizaki, Y.; Tanaka, A.; Muto, Y.; Yokoyama, S.

Citation:

Citation: Kuwasako, Kanako; Takahashi, Mari; Tochio, Naoya; Abe, Chikage; Tsuda, Kengo; Inoue, Makoto; Terada, Takaho; Shirouzu, Mikako; Kobayashi, Naohiro; Kigawa, Takanori; Taguchi, Seiichi; Tanaka, Akiko; Hayashizaki, Yoshihide; Guntert, Peter; Muto, Yutaka; Yokoyama, Shigeyuki. "Solution structure of the second RNA recognition motif (RRM) domain of murine T cell intracellular antigen-1 (TIA-1) and its RNA recognition mode." Biochemistry 47, 6437-6450 (2008).
PubMed: 18500819

Assembly members:

Assembly members:
RRM domain, polymer, 115 residues, Formula weight is not available
RNA (UUUUU), polymer, 5 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RRM domain: GSSGSSGQKKDTSNHFHVFV GDLSPEITTEDIKAAFAPFG RISDARVVKDMATGKSKGYG FVSFFNKWDAENAIQQMGGQ WLGGRQIRTNWATRKPPAPK STYESNTKQSGPSSG
RNA (UUUUU): UUUUU

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
15N chemical shifts	100
1H chemical shifts	100

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RRM domain	1
2	RNA (UUUUU)	2

Entities:

Entity 1, RRM domain 115 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLN

LYS

2

ASP

THR

SER

ASN

HIS

PHE

HIS

VAL

PHE

VAL

3

GLY

ASP

LEU

SER

PRO

GLU

ILE

THR

GLU

4

ASP

ILE

LYS

ALA

PHE

ALA

PRO

PHE

GLY

5

ARG

ILE

SER

ASP

ALA

ARG

VAL

LYS

ASP

6

MET

ALA

THR

GLY

LYS

SER

LYS

GLY

TYR

GLY

7

PHE

VAL

SER

PHE

ASN

LYS

TRP

ASP

ALA

8

GLU

ASN

ALA

ILE

GLN

MET

GLY

GLN

9

TRP

LEU

GLY

ARG

GLN

ILE

ARG

THR

ASN

10

TRP

ALA

THR

ARG

LYS

PRO

ALA

PRO

LYS

11

SER

THR

TYR

GLU

SER

ASN

THR

LYS

GLN

SER

12

GLY

PRO

SER

GLY

Entity 2, RNA (UUUUU) 5 residues - Formula weight is not available

1

U

Samples:

sample_1: RRM domain, [U-99% 13C; U-99% 15N], 1 mM; RNA (UUUUU) 0.583 mM; TRIS, [U-99% 2H], 20 mM; sodium chloride 100 mM; DTT, [U-98% 2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	condition_1
3D 1H-13C NOESY	sample_1	isotropic	condition_1

Software:

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement, structure solution

NMR spectrometers:

Bruker Avance 800 MHz

BMRB	11370 11371 11372 11373 11374 11376
PDB	2DGO 2RNE
GB	EAW99825
REF	XP_005430921