BMRB Entry 11366

Name: Assigned chemical shifts of the human spliceosomal protein complex p14-SF3b155
Published: 2011-09-07

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PDB ID: 2fho
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11366
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assigned chemical shifts of the human spliceosomal protein complex p14-SF3b155

Deposition date:

2010-09-08

Original release date:

2011-09-07

Authors:

Kuwasako, K.; Dohmae, N.; Inoue, M.; Shirouzu, M.; Guntert, P.; Seraphin, B.; Muto, Y.; Yokoyama, S.

Citation:

Citation: Kuwasako, K.; Dohmae, N.; Inoue, M.; Shirouzu, M.; Guntert, P.; Seraphin, B.; Muto, Y.; Yokoyama, S.. "NMR solution structure of the human spliceosomal protein complex p14-SF3b155" .

Assembly members:

Assembly members:
spliceosomal protein SF3b155, residues in database 379-424, polymer, 47 residues, Formula weight is not available
spliceosomal protein p14, RNA recognition motif, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: Escherichia coli Vector: PGEX6P-1-SF3B155(379-424)-HIS6-P14(8-93)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
spliceosomal protein SF3b155, residues in database 379-424: GTPEQLQAWRWEREIDERNR PLSDEELDAMFPEGYKVLPP PAGYVPI
spliceosomal protein p14, RNA recognition motif: GRANIRLPPEVNRILYIRNL PYKITAEEMYDIFGKYGPIR QIRVGNTPETRGTAYVVYED IFDAKNACDHLSGFNVCNRY LVVLYYN

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	516
15N chemical shifts	112
1H chemical shifts	804

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	spliceosomal protein SF3b155, residues in database 379-424	1
2	spliceosomal protein p14, RNA recognition motif	2

Entities:

Entity 1, spliceosomal protein SF3b155, residues in database 379-424 47 residues - Formula weight is not available

1

GLY

THR

PRO

GLU

GLN

LEU

GLN

ALA

TRP

ARG

2

TRP

GLU

ARG

GLU

ILE

ASP

GLU

ARG

ASN

ARG

3

PRO

LEU

SER

ASP

GLU

LEU

ASP

ALA

MET

4

PHE

PRO

GLU

GLY

TYR

LYS

VAL

LEU

PRO

5

PRO

ALA

GLY

TYR

VAL

PRO

ILE

Entity 2, spliceosomal protein p14, RNA recognition motif 87 residues - Formula weight is not available

1

GLY

ARG

ALA

ASN

ILE

ARG

LEU

PRO

GLU

2

VAL

ASN

ARG

ILE

LEU

TYR

ILE

ARG

ASN

LEU

3

PRO

TYR

LYS

ILE

THR

ALA

GLU

MET

TYR

4

ASP

ILE

PHE

GLY

LYS

TYR

GLY

PRO

ILE

ARG

5

GLN

ILE

ARG

VAL

GLY

ASN

THR

PRO

GLU

THR

6

ARG

GLY

THR

ALA

TYR

VAL

TYR

GLU

ASP

7

ILE

PHE

ASP

ALA

LYS

ASN

ALA

CYS

ASP

HIS

8

LEU

SER

GLY

PHE

ASN

VAL

CYS

ASN

ARG

TYR

9

LEU

VAL

LEU

TYR

ASN

Samples:

sample_1: spliceosomal protein SF3b155, residues in database 379-424 300 mM; spliceosomal protein p14, RNA recognition motif 300 mM; Sodium Phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F - processing

NMRView v5.0.4, Johnson, B.A - data analysis

Kujira v0.8995, Kobayashi, N - data analysis

CYANA v2.1, Guntert, P - refinement, structure solution

OPALP v1.4, Koradi, R. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

BMRB	11367
PDB	2F9D 2F9J 2FHO 3LQV 2F9D 2F9J 2FHO 3LQV
DBJ	BAB28369 BAB40140 BAE24537 BAE27592 BAB22052 BAB22936 BAE30577 BAG34849 BAI45933
EMBL	CAA70201 CAG00132 CAJ82723 CDQ59950 CDQ82948
GB	AAC97189 AAG01404 AAH03419 AAH15530 AAH28959 AAD34105 AAF29138 AAG09698 AAH15463 AAH19535
REF	NP_001084150 NP_001179923 NP_001252892 NP_036565 NP_112456 NP_001002067 NP_001017249 NP_001039482 NP_001079940 NP_001153997
SP	O57683 O75533 Q99NB9 P59708 Q9Y3B4
TPG	DAA32571 DAA21891 DAA24388
AlphaFold	O57683 O75533 Q99NB9 P59708 Q9Y3B4