BMRB Entry 11364

Name: Solution structure of the first SURP domain of human splicing factor SF3a120
Published: 2011-09-07

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PDB ID: 2dt6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11364
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the first SURP domain of human splicing factor SF3a120

Deposition date:

2010-09-07

Original release date:

2011-09-07

Authors:

Kuwasako, K.; He, F.; Inoue, M.; Tanaka, A.; Sugano, S.; Guntert, P.; Muto, Y.; Yokoyama, S.

Citation:

Citation: Kuwasako, K.; He, F.; Inoue, M.; Tanaka, A.; Sugano, S.; Guntert, P.; Muto, Y.; Yokoyama, S.. "Solution structures of the SURP domains and the subunit-assembly mechanism within the splicing factor SF3a complex in 17S U2 snRNP" Structure ., .-..

Assembly members:

Assembly members:
SURP domain, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: Escherichia coli Vector: pET15b-SF3a120 SURP1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SURP domain: GEVRNIVDKTASFVARNGPE FEARIRQNEINNPKFNFLNP NDPYHAYYRHKVSEFKEGKA QEPS

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	291
15N chemical shifts	66
1H chemical shifts	449

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SURP domain	1

Entities:

Entity 1, SURP domain 64 residues - Formula weight is not available

1

GLY

GLU

VAL

ARG

ASN

ILE

VAL

ASP

LYS

THR

2

ALA

SER

PHE

VAL

ALA

ARG

ASN

GLY

PRO

GLU

3

PHE

GLU

ALA

ARG

ILE

ARG

GLN

ASN

GLU

ILE

4

ASN

PRO

LYS

PHE

ASN

PHE

LEU

ASN

PRO

5

ASN

ASP

PRO

TYR

HIS

ALA

TYR

ARG

HIS

6

LYS

VAL

SER

GLU

PHE

LYS

GLU

GLY

LYS

ALA

7

GLN

GLU

PRO

SER

Samples:

sample_1: SURP domain, [U-13C; U-15N], 2.0 mM; soldium phosphate 20 mM; d-DTT 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 20 mM; pH: 7.0; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9321, Kobayashi, N. - data analysis

CYANA v2.1, Guntert, P. - refinement, structure solution

OPALP v1.4, Koradi, R. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 700 MHz
Bruker AVANCE 800 MHz

PDB	2DT6
REF	XP_005961296 XP_007461252 XP_012967930