BMRB Entry 11360
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11360
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: He, Fahu; Umehara, Takashi; Saito, Kohei; Harada, Takushi; Watanabe, Satoru; Yabuki, Takashi; Kigawa, Takanori; Takahashi, Mari; Kuwasako, Kanako; Tsuda, Kengo; Matsuda, Takayoshi; Aoki, Masaaki; Seki, Eiko; Kobayashi, Naohiro; Guntert, Peter; Yokoyama, Shigeyuki; Muto, Yutaka. "Structural insight into the zinc finger CW domain as a histone modification reader." Structure 18, 1127-1139 (2010).
PubMed: 20826339
Assembly members:
zf-CW domain, polymer, 69 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
H3 trimethyl K4 peptide, polymer, 20 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060116-12
Entity Sequences (FASTA):
zf-CW domain: GSSGSSGEISGFGQCLVWVQ
CSFPNCGKWRRLCGNIDPSV
LPDNWSCDQNTDVQYNRCDI
PEETWTGLE
H3 trimethyl K4 peptide: ARTXQTARKSTGGKAPRKQL
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 57 |
| 1H chemical shifts | 57 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | zf-CW domain | 1 |
| 2 | ZINC ION | 2 |
| 3 | H3 trimethyl K4 peptide | 3 |
Entities:
Entity 1, zf-CW domain 69 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLU | ILE | SER | ||||
| 2 | GLY | PHE | GLY | GLN | CYS | LEU | VAL | TRP | VAL | GLN | ||||
| 3 | CYS | SER | PHE | PRO | ASN | CYS | GLY | LYS | TRP | ARG | ||||
| 4 | ARG | LEU | CYS | GLY | ASN | ILE | ASP | PRO | SER | VAL | ||||
| 5 | LEU | PRO | ASP | ASN | TRP | SER | CYS | ASP | GLN | ASN | ||||
| 6 | THR | ASP | VAL | GLN | TYR | ASN | ARG | CYS | ASP | ILE | ||||
| 7 | PRO | GLU | GLU | THR | TRP | THR | GLY | LEU | GLU |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Entity 3, H3 trimethyl K4 peptide 20 residues - Formula weight is not available
| 1 | ALA | ARG | THR | M3L | GLN | THR | ALA | ARG | LYS | SER | |
| 2 | THR | GLY | GLY | LYS | ALA | PRO | ARG | LYS | GLN | LEU |
Samples:
sample_1: zf-CW domain mM; H3 trimethyl K4 peptide mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 50 uM; IDA 1 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9819, Kobayashi, N. - data analysis
CYANA v2.1, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| BMRB | 11115 11358 11359 11361 11362 11359 |
| PDB | |
| DBJ | BAA91424 BAC04028 BAG62414 BAG63489 |
| EMBL | CAB66669 CAT02683 CAT02685 CAT02686 CAT02687 CAT02689 |
| GB | AAH02725 AIC60275 EAW76538 EAW76539 EAW76540 AAT28135 AAT28136 AAT28137 AAT28138 AAT28139 |
| REF | NP_001244937 NP_060454 XP_002803268 XP_003318693 XP_003318694 XP_001194483 XP_002028918 XP_002904927 XP_002927153 XP_003502247 |
| SP | Q9H0M4 |
| AlphaFold | Q9H0M4 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated peaks