BMRB Entry 11355

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PDB ID: 2dk4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11355
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of Splicing Factor Motif in Pre-mRNA splicing factor 18 (hPRP18)

Deposition date:

2010-09-07

Original release date:

2011-09-07

Authors:

He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.

Citation:

Citation: He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.. "Solution structure of Splicing Factor Motif in Pre-mRNA splicing factor 18 (hPRP18)" .

Assembly members:

Assembly members:
SFM domain, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050801-10

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SFM domain: GSSGSSGTSSNPVLELELAE EKLPMTLSRQEVIRRLRERG EPIRLFGETDYDAFQRLRKI EILTPEVNKGSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	309
15N chemical shifts	65
1H chemical shifts	494

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SFM domain	1

Entities:

Entity 1, SFM domain 76 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

THR

SER

2

ASN

PRO

VAL

LEU

GLU

LEU

GLU

LEU

ALA

GLU

3

GLU

LYS

LEU

PRO

MET

THR

LEU

SER

ARG

GLN

4

GLU

VAL

ILE

ARG

LEU

ARG

GLU

ARG

GLY

5

GLU

PRO

ILE

ARG

LEU

PHE

GLY

GLU

THR

ASP

6

TYR

ASP

ALA

PHE

GLN

ARG

LEU

ARG

LYS

ILE

7

GLU

ILE

LEU

THR

PRO

GLU

VAL

ASN

LYS

GLY

8

SER

GLY

PRO

SER

GLY

Samples:

sample_1: SFM domain, [U-13C; U-15N], 0.8 mM; phosphate buffer NA 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio F. - processing

NMRView v5.0.4, Johnson B.A. - data analysis

Kujira v0.9321, Kobayashi N. - data analysis

CYANA v1.0.8, Guntert P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2DK4
DBJ	BAC28933 BAE22619 BAE22717 BAE24112 BAG37337
EMBL	CAH90004
GB	AAB41490 AAF44715 AAH00794 AAH04573 AAH58631
REF	NP_001068791 NP_001124950 NP_001231788 NP_001247713 NP_001296236
SP	Q2HJ41 Q5RE03 Q8BM39 Q99633 Q9JKB8
TPG	DAA23561
AlphaFold	Q2HJ41 Q5RE03 Q8BM39 Q99633 Q9JKB8