BMRB Entry 11351

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PDB ID: 2yue
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11351
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the NEUZ (NHR) domain in Neuralized from Drosophila melanogaster

Deposition date:

2010-09-07

Original release date:

2011-09-07

Authors:

He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Tarada, T.; Yokoyama, S.

Citation:

Citation: He, F.; Saito, K.; Kobayashi, N.; Harada, T.; Watanabe, S.; Kigawa, T.; Guntert, P.; Ohara, O.; Tanaka, A.; Unzai, S.; Muto, Y.; Yokoyama, S.. "Structural and functional characterization of the NHR1 domain of the Drosophila neuralized E3 ligase in the notch signaling pathway." J. Mol. Biol. ., .-..

Assembly members:

Assembly members:
NEUZ(NHR) domain, polymer, 168 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P061002-04

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NEUZ(NHR) domain: GSSGSSGPLQFHSVHGDNIR ISRDGTLARRFESFCRAITF SARPVRINERICVKFAEISN NWNGGIRFGFTSNDPVTLEG TLPKYACPDLTNRPGFWAKA LHEQYCEKDNILYYYVNGAG DVIYGINNEEKGVILTGIDT RSLLWTVIDIYGNCTGIEFL DSRIYMYQ

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	733
15N chemical shifts	173
1H chemical shifts	1144

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NEUZ(NHR) domain	1

Entities:

Entity 1, NEUZ(NHR) domain 168 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

LEU

GLN

2

PHE

HIS

SER

VAL

HIS

GLY

ASP

ASN

ILE

ARG

3

ILE

SER

ARG

ASP

GLY

THR

LEU

ALA

ARG

4

PHE

GLU

SER

PHE

CYS

ARG

ALA

ILE

THR

PHE

5

SER

ALA

ARG

PRO

VAL

ARG

ILE

ASN

GLU

ARG

6

ILE

CYS

VAL

LYS

PHE

ALA

GLU

ILE

SER

ASN

7

ASN

TRP

ASN

GLY

ILE

ARG

PHE

GLY

PHE

8

THR

SER

ASN

ASP

PRO

VAL

THR

LEU

GLU

GLY

9

THR

LEU

PRO

LYS

TYR

ALA

CYS

PRO

ASP

LEU

10

THR

ASN

ARG

PRO

GLY

PHE

TRP

ALA

LYS

ALA

11

LEU

HIS

GLU

GLN

TYR

CYS

GLU

LYS

ASP

ASN

12

ILE

LEU

TYR

VAL

ASN

GLY

ALA

GLY

13

ASP

VAL

ILE

TYR

GLY

ILE

ASN

GLU

14

LYS

GLY

VAL

ILE

LEU

THR

GLY

ILE

ASP

THR

15

ARG

SER

LEU

TRP

THR

VAL

ILE

ASP

ILE

16

TYR

GLY

ASN

CYS

THR

GLY

ILE

GLU

PHE

LEU

17

ASP

SER

ARG

ILE

TYR

MET

TYR

GLN

Samples:

sample_1: NEUZ(NHR) domain, [U-13C; U-15N], 1.0 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.1, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz