BMRB Entry 11348

Name: Solution structure of the NEUZ domain in KIAA1787 protein
Published: 2011-09-07

Click here to enlarge.

PDB ID: 2e63
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11348
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of the NEUZ domain in KIAA1787 protein

Deposition date:

2010-09-07

Original release date:

2011-09-07

Authors:

He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.

Citation:

Citation: He, F.; Saito, K.; Kobayashi, N.; Harada, T.; Watanabe, S.; Kigawa, T.; Guntert, P.; Ohara, O.; Tanaka, A.; Unzai, S.; Muto, Y.; Yokoyama, S.. "Structural and functional characterization of the NHR1 domain of the Drosophila neuralized E3 ligase in the notch signaling pathway." J. Mol. Biol. ., .-..

Assembly members:

Assembly members:
neuralized domain, polymer, 170 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060508-08

Entity Sequences (FASTA):

Entity Sequences (FASTA):
neuralized domain: GSSGSSGELHPRTGRLVSLS ACGRTARRQQPGQEFNHGLV LSREPLRDGRVFTVRIDRKV NSWSGSIEIGVTALDPSVLD FPSSATGLKGGSWVVSGCSV LRDGRSVLEEYGQDLDQLGE GDRVGVERTVAGELRLWVNG RDCGVAATGLPPRVWAVVDL YGKCTQITVL

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	706
15N chemical shifts	170
1H chemical shifts	1129

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	neuralized domain	1

Entities:

Entity 1, neuralized domain 170 residues - Formula weight is not available

1	GLY	SER	SER	GLY	SER	SER	GLY	GLU	LEU	HIS
2	PRO	ARG	THR	GLY	ARG	LEU	VAL	SER	LEU	SER
3	ALA	CYS	GLY	ARG	THR	ALA	ARG	ARG	GLN	GLN
4	PRO	GLY	GLN	GLU	PHE	ASN	HIS	GLY	LEU	VAL
5	LEU	SER	ARG	GLU	PRO	LEU	ARG	ASP	GLY	ARG
6	VAL	PHE	THR	VAL	ARG	ILE	ASP	ARG	LYS	VAL
7	ASN	SER	TRP	SER	GLY	SER	ILE	GLU	ILE	GLY
8	VAL	THR	ALA	LEU	ASP	PRO	SER	VAL	LEU	ASP
9	PHE	PRO	SER	SER	ALA	THR	GLY	LEU	LYS	GLY
10	GLY	SER	TRP	VAL	VAL	SER	GLY	CYS	SER	VAL
11	LEU	ARG	ASP	GLY	ARG	SER	VAL	LEU	GLU	GLU
12	TYR	GLY	GLN	ASP	LEU	ASP	GLN	LEU	GLY	GLU
13	GLY	ASP	ARG	VAL	GLY	VAL	GLU	ARG	THR	VAL
14	ALA	GLY	GLU	LEU	ARG	LEU	TRP	VAL	ASN	GLY
15	ARG	ASP	CYS	GLY	VAL	ALA	ALA	THR	GLY	LEU
16	PRO	PRO	ARG	VAL	TRP	ALA	VAL	VAL	ASP	LEU
17	TYR	GLY	LYS	CYS	THR	GLN	ILE	THR	VAL	LEU

Samples:

sample_1: neuralized domain, [U-13C; U-15N], 1.0 ± 0.1 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio F. - processing

NMRView v5.0.4, Johnson B.A. - data analysis

Kujira v0.9819, Kobayashi N. - data analysis

CYANA v2.1, Guntert P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

1	GLY	SER	SER	GLY	SER	SER	GLY	GLU	LEU	HIS
2	PRO	ARG	THR	GLY	ARG	LEU	VAL	SER	LEU	SER
3	ALA	CYS	GLY	ARG	THR	ALA	ARG	ARG	GLN	GLN
4	PRO	GLY	GLN	GLU	PHE	ASN	HIS	GLY	LEU	VAL
5	LEU	SER	ARG	GLU	PRO	LEU	ARG	ASP	GLY	ARG
6	VAL	PHE	THR	VAL	ARG	ILE	ASP	ARG	LYS	VAL
7	ASN	SER	TRP	SER	GLY	SER	ILE	GLU	ILE	GLY
8	VAL	THR	ALA	LEU	ASP	PRO	SER	VAL	LEU	ASP
9	PHE	PRO	SER	SER	ALA	THR	GLY	LEU	LYS	GLY
10	GLY	SER	TRP	VAL	VAL	SER	GLY	CYS	SER	VAL
11	LEU	ARG	ASP	GLY	ARG	SER	VAL	LEU	GLU	GLU
12	TYR	GLY	GLN	ASP	LEU	ASP	GLN	LEU	GLY	GLU
13	GLY	ASP	ARG	VAL	GLY	VAL	GLU	ARG	THR	VAL
14	ALA	GLY	GLU	LEU	ARG	LEU	TRP	VAL	ASN	GLY
15	ARG	ASP	CYS	GLY	VAL	ALA	ALA	THR	GLY	LEU
16	PRO	PRO	ARG	VAL	TRP	ALA	VAL	VAL	ASP	LEU
17	TYR	GLY	LYS	CYS	THR	GLN	ILE	THR	VAL	LEU

1	GLY	SER	SER	GLY	SER	SER	GLY	GLU	LEU	HIS
2	PRO	ARG	THR	GLY	ARG	LEU	VAL	SER	LEU	SER
3	ALA	CYS	GLY	ARG	THR	ALA	ARG	ARG	GLN	GLN
4	PRO	GLY	GLN	GLU	PHE	ASN	HIS	GLY	LEU	VAL
5	LEU	SER	ARG	GLU	PRO	LEU	ARG	ASP	GLY	ARG
6	VAL	PHE	THR	VAL	ARG	ILE	ASP	ARG	LYS	VAL
7	ASN	SER	TRP	SER	GLY	SER	ILE	GLU	ILE	GLY
8	VAL	THR	ALA	LEU	ASP	PRO	SER	VAL	LEU	ASP
9	PHE	PRO	SER	SER	ALA	THR	GLY	LEU	LYS	GLY
10	GLY	SER	TRP	VAL	VAL	SER	GLY	CYS	SER	VAL
11	LEU	ARG	ASP	GLY	ARG	SER	VAL	LEU	GLU	GLU
12	TYR	GLY	GLN	ASP	LEU	ASP	GLN	LEU	GLY	GLU
13	GLY	ASP	ARG	VAL	GLY	VAL	GLU	ARG	THR	VAL
14	ALA	GLY	GLU	LEU	ARG	LEU	TRP	VAL	ASN	GLY
15	ARG	ASP	CYS	GLY	VAL	ALA	ALA	THR	GLY	LEU
16	PRO	PRO	ARG	VAL	TRP	ALA	VAL	VAL	ASP	LEU
17	TYR	GLY	LYS	CYS	THR	GLN	ILE	THR	VAL	LEU