BMRB Entry 11342

Name: Solution Structure of the RING domain of the human RING-box protein 2
Published: 2011-08-19

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PDB ID: 2ecl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11342
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the RING domain of the human RING-box protein 2

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the RING domain of the human RING-box protein 2" .

Assembly members:

Assembly members:
RING domain, polymer, 81 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060828-18

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RING domain: GSSGSSGMWSWDVECDTCAI CRVQVMDACLRCQAENKQED CVVVWGECNHSFHNCCMSLW VKQNNRCPLCQQDWVVQRIG K

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	329
15N chemical shifts	91
1H chemical shifts	522

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RING domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2
4	ZINC ION no.3	2

Entities:

Entity 1, RING domain 81 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

TRP

SER

2

TRP

ASP

VAL

GLU

CYS

ASP

THR

CYS

ALA

ILE

3

CYS

ARG

VAL

GLN

VAL

MET

ASP

ALA

CYS

LEU

4

ARG

CYS

GLN

ALA

GLU

ASN

LYS

GLN

GLU

ASP

5

CYS

VAL

TRP

GLY

GLU

CYS

ASN

HIS

6

SER

PHE

HIS

ASN

CYS

MET

SER

LEU

TRP

7

VAL

LYS

GLN

ASN

ARG

CYS

PRO

LEU

CYS

8

GLN

ASP

TRP

VAL

GLN

ARG

ILE

GLY

9

LYS

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RING domain, [U-13C; U-15N], 1.11 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE II 900 MHz

PDB	2ECL
DBJ	BAB22666 BAE26843 BAG34921 BAJ20342
EMBL	CAF94886 CAG32193 CDQ67670 CDQ72674
GB	AAD25961 AAD25962 AAD30147 AAD55984 AAH05966
REF	NP_001012516 NP_001026478 NP_001069188 NP_001100318 NP_001134211
SP	Q9UBF6 Q9WTZ1
TPG	DAA33088
AlphaFold	Q9UBF6 Q9WTZ1