BMRB Entry 11340

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PDB ID: 2eci
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11340
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the RING domain of the human TNF receptor-associated factor 6 protein

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the RING domain of the human TNF receptor-associated factor 6 protein" .

Assembly members:

Assembly members:
RING domain, polymer, 86 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060613-08

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RING domain: GSSGSSGMEEIQGYDVEFDP PLESKYECPICLMALREAVQ TPCGHRFCKACIIKSIRDAG HKCPVDNEILLENQLFPDNF AKREIL

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	373
15N chemical shifts	80
1H chemical shifts	584

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RING domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, RING domain 86 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

GLU

2

ILE

GLN

GLY

TYR

ASP

VAL

GLU

PHE

ASP

PRO

3

PRO

LEU

GLU

SER

LYS

TYR

GLU

CYS

PRO

ILE

4

CYS

LEU

MET

ALA

LEU

ARG

GLU

ALA

VAL

GLN

5

THR

PRO

CYS

GLY

HIS

ARG

PHE

CYS

LYS

ALA

6

CYS

ILE

LYS

SER

ILE

ARG

ASP

ALA

GLY

7

HIS

LYS

CYS

PRO

VAL

ASP

ASN

GLU

ILE

LEU

8

LEU

GLU

ASN

GLN

LEU

PHE

PRO

ASP

ASN

PHE

9

ALA

LYS

ARG

GLU

ILE

LEU

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RING domain, [U-13C; U-15N], 1.08 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

BMRB	15014
PDB	2ECI 2JMD 3HCS 3HCT 3HCU
GB	AAI02523 ABC47877 ABD72516 ABF06558 AFG21868
REF	NP_001029833 XP_004016459 XP_004264064 XP_004330939 XP_004627096
SP	Q3ZCC3
TPG	DAA21840
AlphaFold	Q3ZCC3