BMRB Entry 11339

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PDB ID: 2ecg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11339
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the ring domain of the Baculoviral IAP repeat-containing protein 4 from Homo sapiens

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the ring domain of the Baculoviral IAP repeat-containing protein 4 from Homo sapiens" .

Assembly members:

Assembly members:
RING domain, polymer, 75 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060417-22

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RING domain: GSSGSSGSLQKEISTEEQLR RLQEEKLCKICMDRNIAIVF VPCGHLVTCKQCAEAVDKCP MCYTVITFKQKIFMS

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	321
15N chemical shifts	75
1H chemical shifts	519

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RING domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, RING domain 75 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

SER

LEU

GLN

2

LYS

GLU

ILE

SER

THR

GLU

GLN

LEU

ARG

3

ARG

LEU

GLN

GLU

LYS

LEU

CYS

LYS

ILE

4

CYS

MET

ASP

ARG

ASN

ILE

ALA

ILE

VAL

PHE

5

VAL

PRO

CYS

GLY

HIS

LEU

VAL

THR

CYS

LYS

6

GLN

CYS

ALA

GLU

ALA

VAL

ASP

LYS

CYS

PRO

7

MET

CYS

TYR

THR

VAL

ILE

THR

PHE

LYS

GLN

8

LYS

ILE

PHE

MET

SER

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RING domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - refinement

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2ECG
DBJ	BAG36609 BAI46620
GB	AAC50373 AAC50518 AAH32729 AAW62257 AAX29953
REF	NP_001158 NP_001191330 XP_003262340 XP_003779771 XP_003823173
SP	P98170
AlphaFold	P98170