BMRB Entry 11337

Name: Solution structure of the RWD domain of human RWD domain containing protein 3
Published: 2011-08-19

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PDB ID: 2ebk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11337
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the RWD domain of human RWD domain containing protein 3

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Yoneyama, M.; Koshiba, S.; Watabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Yoneyama, M.; Koshiba, S.; Watabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the RWD domain of human RWD domain containing protein 3" .

Assembly members:

Assembly members:
RWD domain, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060313-08

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RWD domain: GSSGSSGMAEPVQEELSVLA AIFCRPHEWEVLSRSETDGT VFRIHTKAEGFMDADIPLEL VFHLPVNYPSCLPGISINSE QLTRAQCVTVKEKLLEQAES LLSEPMVHELVLWIQQNLRH ILSQPETG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	555
15N chemical shifts	125
1H chemical shifts	897

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RWD domain	1

Entities:

Entity 1, RWD domain 128 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

ALA

GLU

2

PRO

VAL

GLN

GLU

LEU

SER

VAL

LEU

ALA

3

ALA

ILE

PHE

CYS

ARG

PRO

HIS

GLU

TRP

GLU

4

VAL

LEU

SER

ARG

SER

GLU

THR

ASP

GLY

THR

5

VAL

PHE

ARG

ILE

HIS

THR

LYS

ALA

GLU

GLY

6

PHE

MET

ASP

ALA

ASP

ILE

PRO

LEU

GLU

LEU

7

VAL

PHE

HIS

LEU

PRO

VAL

ASN

TYR

PRO

SER

8

CYS

LEU

PRO

GLY

ILE

SER

ILE

ASN

SER

GLU

9

GLN

LEU

THR

ARG

ALA

GLN

CYS

VAL

THR

VAL

10

LYS

GLU

LYS

LEU

GLU

GLN

ALA

GLU

SER

11

LEU

SER

GLU

PRO

MET

VAL

HIS

GLU

LEU

12

VAL

LEU

TRP

ILE

GLN

ASN

LEU

ARG

HIS

13

ILE

LEU

SER

GLN

PRO

GLU

THR

GLY

Samples:

sample_1: RWD domain, [U-13C; U-15N], 0.63 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2EBK 4Y1L
DBJ	BAF85354 BAF85388 BAG59117 BAI46391
EMBL	CAB43254 CAG38524 CAL37651 CAL38135
GB	AAI25143 AIC56138 AIC63357 EAW73019 EAW73020
PIR	T08730
REF	NP_001121614 NP_001186611 NP_001265176 NP_001265177 NP_056300
SP	Q9Y3V2
AlphaFold	Q9Y3V2