BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11333

Title: Solution structure of the CHY zinc finger domain of the RING finger and CHY zinc finger domain-containing protein 1 from Mus musculus

Deposition date: 2010-08-10 Original release date: 2011-08-19

Authors: Miyamoto, K.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation: Miyamoto, K.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the CHY zinc finger domain of the RING finger and CHY zinc finger domain-containing protein 1 from Mus musculus"  .

Assembly members:
CHY domian, polymer, 143 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: not available   Taxonomy ID: 10090   Superkingdom: Metazoa   Kingdom: Mus   Genus/species: musculus not available

Experimental source:   Production method: cell free synthesis' 'E. coli - cell free' 'E. coli

Entity Sequences (FASTA):
CHY domian: GSSGSSGGVRNLAQGPRGCE HYDRACLLKAPCCDKLYTCR LCHDTNEDHQLDRFKVKEVQ CINCEKLQHAQQTCEDCSTL FGEYYCSICHLFDKDKRQYH CESCGICRIGPKEDFFHCLK CNLCLTTNLRGKHKCIESGP SSG

Data sets:
Data typeCount
13C chemical shifts573
15N chemical shifts137
1H chemical shifts903

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CHY domian1
2ZINC ION no.12
3ZINC ION no.22
4ZINC ION no.32
5ZINC ION no.42
6ZINC ION no.52
7ZINC ION no.62

Entities:

Entity 1, CHY domian 143 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLYVALARG
2   ASNLEUALAGLNGLYPROARGGLYCYSGLU
3   HISTYRASPARGALACYSLEULEULYSALA
4   PROCYSCYSASPLYSLEUTYRTHRCYSARG
5   LEUCYSHISASPTHRASNGLUASPHISGLN
6   LEUASPARGPHELYSVALLYSGLUVALGLN
7   CYSILEASNCYSGLULYSLEUGLNHISALA
8   GLNGLNTHRCYSGLUASPCYSSERTHRLEU
9   PHEGLYGLUTYRTYRCYSSERILECYSHIS
10   LEUPHEASPLYSASPLYSARGGLNTYRHIS
11   CYSGLUSERCYSGLYILECYSARGILEGLY
12   PROLYSGLUASPPHEPHEHISCYSLEULYS
13   CYSASNLEUCYSLEUTHRTHRASNLEUARG
14   GLYLYSHISLYSCYSILEGLUSERGLYPRO
15   SERSERGLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: CHY domain' '[U-13C; U-15N]; natural abundance; natural abundance; natural abundance; natural abundance; natural abundance; .; .

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9732, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts