BMRB Entry 11332

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PDB ID: 2djb
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11332
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the RING domain of the human Polycomb group RING finger protein 6

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the RING domain of the human Polycomb group RING finger protein 6" .

Assembly members:

Assembly members:
RING domain, polymer, 72 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050302-15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RING domain: GSSGSSGNLSELTPYILCSI CKGYLIDATTITECLHTFCK SCIVRHFYYSNRCPKCNIVV HQTQPLSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	300
15N chemical shifts	66
1H chemical shifts	460

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RING domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, RING domain 72 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASN

LEU

SER

2

GLU

LEU

THR

PRO

TYR

ILE

LEU

CYS

SER

ILE

3

CYS

LYS

GLY

TYR

LEU

ILE

ASP

ALA

THR

4

ILE

THR

GLU

CYS

LEU

HIS

THR

PHE

CYS

LYS

5

SER

CYS

ILE

VAL

ARG

HIS

PHE

TYR

SER

6

ASN

ARG

CYS

PRO

LYS

CYS

ASN

ILE

VAL

7

HIS

GLN

THR

GLN

PRO

LEU

SER

GLY

PRO

SER

8

SER

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RING domain, [U-13C; U-15N], 0.43 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9732, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 900 MHz

PDB	2DJB
DBJ	BAB40779 BAB40780 BAE21174 BAF83368 BAJ17816
GB	AAH10235 AAH16195 AAH83820 AAH89460 ABZ92150
REF	NP_001011663 NP_001013172 NP_001181540 NP_001192549 NP_081930
SP	Q5XI70 Q99NA9 Q9BYE7
TPG	DAA14916
AlphaFold	Q5XI70 Q99NA9 Q9BYE7