BMRB Entry 11331

Title:
Solution structure of the B-box domain of the human Midline-2 protein
Deposition date:
2010-08-10
Original release date:
2011-08-19
Authors:
Miyamoto, K.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation:

Citation: Miyamoto, K.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the B-box domain of the human Midline-2 protein"  .

Assembly members:

Assembly members:
zf-B box, polymer, 84 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050302-80

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts329
15N chemical shifts79
1H chemical shifts534

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1zf-B box1
2ZINC ION no.12
3ZINC ION no.22

Entities:

Entity 1, zf-B box 84 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYVALGLUPRO
2   VALPROASPTHRHISLEUARGGLYILETHR
3   CYSLEUASPHISGLUASNGLULYSVALASN
4   METTYRCYSVALSERASPASPGLNLEUILE
5   CYSALALEUCYSLYSLEUVALGLYARGHIS
6   ARGASPHISGLNVALALASERLEUASNASP
7   ARGPHEGLULYSLEULYSGLNTHRLEUGLU
8   METASNLEUTHRASNLEUVALLYSSERGLY
9   PROSERSERGLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: zf-B box, [U-13C; U-15N], 1.92 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9732, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

PDB
EMBL CAB56154 CAB56170
GB AAF07340 AAF07341 AAH17707 AAI40362 AAI46505
REF NP_001178818 NP_035975 NP_036348 NP_438112 XP_001091577
SP Q9QUS6 Q9UJV3
AlphaFold Q9QUS6 Q9UJV3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks