BMRB Entry 11329

Title:
Solution structure of the Bromodomain of human SWI/SNF related matrix associated actin dependent regulator of cromatin subfamily A member 2
Deposition date:
2010-08-10
Original release date:
2011-08-19
Authors:
Yoneyama, M.; Kigawa, T.; Saito, K.; Tochio, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation:

Citation: Yoneyama, M.; Kigawa, T.; Saito, K.; Tochio, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the Bromodomain of human SWI/SNF related matrix associated actin dependent regulator of cromatin subfamily A member 2"  .

Assembly members:

Assembly members:
Bromodomain, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050302-31

Data sets:
Data typeCount
13C chemical shifts528
15N chemical shifts115
1H chemical shifts847

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Bromodomain1

Entities:

Entity 1, Bromodomain 123 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYSERPROASN
2   PROPROLYSLEUTHRLYSGLNMETASNALA
3   ILEILEASPTHRVALILEASNTYRLYSASP
4   SERSERGLYARGGLNLEUSERGLUVALPHE
5   ILEGLNLEUPROSERARGLYSGLULEUPRO
6   GLUTYRTYRGLULEUILEARGLYSPROVAL
7   ASPPHELYSLYSILELYSGLUARGILEARG
8   ASNHISLYSTYRARGSERLEUGLYASPLEU
9   GLULYSASPVALMETLEULEUCYSHISASN
10   ALAGLNTHRPHEASNLEUGLUGLYSERGLN
11   ILETYRGLUASPSERILEVALLEUGLNSER
12   VALPHELYSSERALAARGGLNSERGLYPRO
13   SERSERGLY

Samples:

sample_1: Bromodomain, [U-13C; U-15N], 1.39 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; Guanidine oxalate 75 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 195 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9318, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA05142 BAB27925 BAC04280 BAE90149 BAG60343
EMBL CAA62832 CAH89596
GB AAH22975 AAH56199 AAH75641 AAI42522 AAI56186
REF NP_001092585 NP_001124708 NP_001276326 NP_001276327 NP_001276328
SP Q6DIC0
TPG DAA26902
AlphaFold Q6DIC0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks