BMRB Entry 11327

Click here to enlarge.

PDB ID: 2d8u
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11327
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of the B-box domain of the human tripartite motif-containing 63 protein

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the B-box domain of the human tripartite motif-containing 63 protein" .

Assembly members:

Assembly members:
zf-B box, polymer, 64 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050302-45

Entity Sequences (FASTA):

Entity Sequences (FASTA):
zf-B box: GSSGSSGHPMCKEHEDEKIN IYCLTCEVPTCSMCKVFGIH KACEVAPLQSVFQGQKTESG PSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	243
15N chemical shifts	54
1H chemical shifts	377

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	zf-B box	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, zf-B box 64 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

HIS

PRO

MET

2

CYS

LYS

GLU

HIS

GLU

ASP

GLU

LYS

ILE

ASN

3

ILE

TYR

CYS

LEU

THR

CYS

GLU

VAL

PRO

THR

4

CYS

SER

MET

CYS

LYS

VAL

PHE

GLY

ILE

HIS

5

LYS

ALA

CYS

GLU

VAL

ALA

PRO

LEU

GLN

SER

6

VAL

PHE

GLN

GLY

GLN

LYS

THR

GLU

SER

GLY

7

PRO

SER

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: zf-B_box, [U-13C; U-15N], 2.09 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9321, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 700 MHz

PDB	2D8U
DBJ	BAB71318 BAG60157
EMBL	CAC33173 CAC81706
GB	AAH80529 AAI12722 AAK39519 AAP36037 AAP36890
REF	NP_001039760 NP_001171685 NP_115977 XP_002811293 XP_002811294
SP	Q969Q1
TPG	DAA32118
AlphaFold	Q969Q1