BMRB Entry 11326

Name: Solution structure of the RING domain of the human RING finger protein 146
Published: 2011-08-19

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PDB ID: 2d8t
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11326
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the RING domain of the human RING finger protein 146

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the RING domain of the human RING finger protein 146" .

Assembly members:

Assembly members:
RING domain, polymer, 71 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050302-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RING domain: GSSGSSGNTAPSLTVPECAI CLQTCVHPVSLPCKHVFCYL CVKGASWLGKRCALCRQEIP EDFLDSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	266
15N chemical shifts	56
1H chemical shifts	422

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RING domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, RING domain 71 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASN

THR

ALA

2

PRO

SER

LEU

THR

VAL

PRO

GLU

CYS

ALA

ILE

3

CYS

LEU

GLN

THR

CYS

VAL

HIS

PRO

VAL

SER

4

LEU

PRO

CYS

LYS

HIS

VAL

PHE

CYS

TYR

LEU

5

CYS

VAL

LYS

GLY

ALA

SER

TRP

LEU

GLY

LYS

6

ARG

CYS

ALA

LEU

CYS

ARG

GLN

GLU

ILE

PRO

7

GLU

ASP

PHE

LEU

ASP

SER

GLY

PRO

SER

8

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RING domain, [U-13C; U-15N], 1.34 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9321, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2D8T 4QPL
DBJ	BAB28015 BAB55108 BAB55196 BAB55359 BAE31549
EMBL	CAB66763 CAC85986 CAG38545 CAH89794 CAH90287
GB	AAH08235 AAH50795 AAH83675 AAI02140 AIC52443
REF	NP_001012060 NP_001070448 NP_001103666 NP_001103667 NP_001103668
SP	E1B7X3 Q2PFU6 Q3T139 Q5REL3 Q5XIK5
TPG	DAA20901 DAA26356
AlphaFold	E1B7X3 Q2PFU6 Q3T139 Q5REL3 Q5XIK5