BMRB Entry 11325

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11325
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution structure of the RING domain of the human cellular modulator of immune recognition protein
Deposition date:
2010-08-10
Original release date:
2011-08-19
Authors:
Miyamoto, K.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation:

Citation: Miyamoto, K.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the RING domain of the human cellular modulator of immune recognition protein"  .

Assembly members:

Assembly members:
RING domain, polymer, 80 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P041123-09

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RING domain: GSSGSSGTSITPSSQDICRI CHCEGDDESPLITPCHCTGS LHFVHQACLQQWIKSSDTRC CELCKYEFIMETKLSGPSSG

Data sets:
Data typeCount
13C chemical shifts299
15N chemical shifts68
1H chemical shifts456

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RING domain1
2ZINC ION no.12
3ZINC ION no.22

Entities:

Entity 1, RING domain 80 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYTHRSERILE
2   THRPROSERSERGLNASPILECYSARGILE
3   CYSHISCYSGLUGLYASPASPGLUSERPRO
4   LEUILETHRPROCYSHISCYSTHRGLYSER
5   LEUHISPHEVALHISGLNALACYSLEUGLN
6   GLNTRPILELYSSERSERASPTHRARGCYS
7   CYSGLULEUCYSLYSTYRGLUPHEILEMET
8   GLUTHRLYSLEUSERGLYPROSERSERGLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: RING domain, [U-13C; U-15N], 1.22 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9321, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAG36144 BAI47281
EMBL CAJ82358
GB AAH25394 AAH66988 AAH74623 AAH84236 AIC58002
REF NP_001002266 NP_001004831 NP_001088241 NP_659458 XP_001500065
SP Q28IK8 Q5T0T0 Q5XH39
AlphaFold Q28IK8 Q5T0T0 Q5XH39

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks