BMRB Entry 11324

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PDB ID: 2d8r
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11324
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the thap domain of the human thap domain-containing protein 2

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Kigawa, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the thap domain of the human thap domain-containing protein 2" .

Assembly members:

Assembly members:
THAP, polymer, 99 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050302-85

Entity Sequences (FASTA):

Entity Sequences (FASTA):
THAP: GSSGSSGMPTNCAAAGCATT YNKHINISFHRFPLDPKRRK EWVRLVRRKNFVPGKHTFLC SKHFEASCFDLTGQTRRLKM DAVPTIFDFCTHISGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	415
15N chemical shifts	81
1H chemical shifts	625

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	THAP	1
2	ZINC ION	2

Entities:

Entity 1, THAP 99 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

PRO

THR

2

ASN

CYS

ALA

GLY

CYS

ALA

THR

3

TYR

ASN

LYS

HIS

ILE

ASN

ILE

SER

PHE

HIS

4

ARG

PHE

PRO

LEU

ASP

PRO

LYS

ARG

LYS

5

GLU

TRP

VAL

ARG

LEU

VAL

ARG

LYS

ASN

6

PHE

VAL

PRO

GLY

LYS

HIS

THR

PHE

LEU

CYS

7

SER

LYS

HIS

PHE

GLU

ALA

SER

CYS

PHE

ASP

8

LEU

THR

GLY

GLN

THR

ARG

LEU

LYS

MET

9

ASP

ALA

VAL

PRO

THR

ILE

PHE

ASP

PHE

CYS

10

THR

HIS

ILE

SER

GLY

PRO

SER

GLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: THAP domain, [U-13C; U-15N], 0.84 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B., A. - data analysis

Kujira v0.9321, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2D8R
DBJ	BAG36240 BAI46412
EMBL	CAB66542 CAG38516 CAH91411 CAL37961
GB	AAH08358 AIC52465 EAW97265 EHH20970 EHH66496
REF	NP_001153224 NP_001180430 NP_113623 XP_002752807 XP_003126431
SP	Q9H0W7
AlphaFold	Q9H0W7