BMRB Entry 11320

Name: solution structure of the sh3 domain-binding glutamic acid-rich-like protein 2
Published: 2011-08-19

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PDB ID: 2ct6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11320
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

solution structure of the sh3 domain-binding glutamic acid-rich-like protein 2

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "solution structure of the sh3 domain-binding glutamic acid-rich-like protein 2" .

Assembly members:

Assembly members:
SH3BGRL2, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040614-04

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3BGRL2: GSSGSSGMVIRVFIASSSGF VAIKKKQQDVVRFLEANKIE FEEVDITMSEEQRQWMYKNV PPEKKPTQGNPLPPQIFNGD RYCGDYDSFFESKESNTVFS FLGLKSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	484
15N chemical shifts	110
1H chemical shifts	755

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SH3BGRL2	1

Entities:

Entity 1, SH3BGRL2 111 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

VAL

ILE

2

ARG

VAL

PHE

ILE

ALA

SER

GLY

PHE

3

VAL

ALA

ILE

LYS

GLN

ASP

VAL

4

VAL

ARG

PHE

LEU

GLU

ALA

ASN

LYS

ILE

GLU

5

PHE

GLU

VAL

ASP

ILE

THR

MET

SER

GLU

6

GLU

GLN

ARG

GLN

TRP

MET

TYR

LYS

ASN

VAL

7

PRO

GLU

LYS

PRO

THR

GLN

GLY

ASN

8

PRO

LEU

PRO

GLN

ILE

PHE

ASN

GLY

ASP

9

ARG

TYR

CYS

GLY

ASP

TYR

ASP

SER

PHE

10

GLU

SER

LYS

GLU

SER

ASN

THR

VAL

PHE

SER

11

PHE

LEU

GLY

LEU

LYS

SER

GLY

PRO

SER

12

GLY

Samples:

sample_1: SH3BGRL2, [U-13C; U-15N], 0.95 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.925, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 900 MHz

PDB	2CT6
DBJ	BAG34700
EMBL	CAC35771 CAH89748
GB	AAH40489 AAH52987 AAH60799 AAH70059 AAI09044
REF	NP_113657 XP_001146260 XP_003258313 XP_003897882 XP_003923315
SP	Q5REQ9 Q9UJC5
AlphaFold	Q5REQ9 Q9UJC5