BMRB Entry 11319

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PDB ID: 2ct5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11319
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the zinc finger BED domain of the zinc finger BED domain containing protein 1

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the zinc finger BED domain of the zinc finger BED domain containing protein 1" .

Assembly members:

Assembly members:
zinc finger BED domain, polymer, 73 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040308-29

Entity Sequences (FASTA):

Entity Sequences (FASTA):
zinc finger BED domain: GSSGSSGSKVWKYFGFDTNA EGCILQWKKIYCRICMAQIA YSGNTSNLSYHLEKNHPEEF CEFVKSNSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	290
15N chemical shifts	66
1H chemical shifts	433

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	zinc finger BED domain	1
2	ZINC ION	2

Entities:

Entity 1, zinc finger BED domain 73 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

SER

LYS

VAL

2

TRP

LYS

TYR

PHE

GLY

PHE

ASP

THR

ASN

ALA

3

GLU

GLY

CYS

ILE

LEU

GLN

TRP

LYS

ILE

4

TYR

CYS

ARG

ILE

CYS

MET

ALA

GLN

ILE

ALA

5

TYR

SER

GLY

ASN

THR

SER

ASN

LEU

SER

TYR

6

HIS

LEU

GLU

LYS

ASN

HIS

PRO

GLU

PHE

7

CYS

GLU

PHE

VAL

LYS

SER

ASN

SER

GLY

PRO

8

SER

GLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: zinc finger BED domain, [U-13C; U-15N], 1.09 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.01 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.925, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2CT5
DBJ	BAA34505 BAG09847
EMBL	CAA76545 CAA76660
GB	AAH15030 ABM84566 ABM86530 AIC55542 EAW98691
REF	NP_001164606 NP_001164607 NP_001253637 NP_004720 XP_002831388
SP	O96006
AlphaFold	O96006