BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11316

Title: Solution Structure of the zinc finger domain of Transcriptional repressor CTCF protein

Deposition date: 2010-08-10 Original release date: 2011-08-19

Authors: Miyamoto, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Miyamoto, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the zinc finger domain of Transcriptional repressor CTCF protein"  .

Assembly members:
zinc finger domain, polymer, 77 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis' 'E. coli - cell free' 'E. coli

Entity Sequences (FASTA):
zinc finger domain: GSSGSSGRTHSGEKPYECYI CHARFTQSGTMKMHILQKHT ENVAKFHCPHCDTVIARKSD LGVHLRKQHSYSGPSSG

Data sets:
Data typeCount
13C chemical shifts298
15N chemical shifts61
1H chemical shifts456

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1zinc finger domain1
2ZINC ION no.12
3ZINC ION no.22

Entities:

Entity 1, zinc finger domain 77 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGTHRHIS
2   SERGLYGLULYSPROTYRGLUCYSTYRILE
3   CYSHISALAARGPHETHRGLNSERGLYTHR
4   METLYSMETHISILELEUGLNLYSHISTHR
5   GLUASNVALALALYSPHEHISCYSPROHIS
6   CYSASPTHRVALILEALAARGLYSSERASP
7   LEUGLYVALHISLEUARGLYSGLNHISSER
8   TYRSERGLYPROSERSERGLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: zinc finger domain' '[U-13C; U-15N]; natural abundance; natural abundance; natural abundance; natural abundance; natural abundance; .; .

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.925, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

PDB
REF XP_010765514

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts