BMRB Entry 11314

Name: Solution Structure of the RING domain of the Synaptotagmin-like protein 4
Published: 2011-08-19

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PDB ID: 2csz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11314
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the RING domain of the Synaptotagmin-like protein 4

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Sato, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Sato, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the RING domain of the Synaptotagmin-like protein 4" .

Assembly members:

Assembly members:
RING domain, polymer, 76 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P041101-16

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RING domain: GSSGSSGLLEIKRKGAKRGS QHYSDRTCARCQESLGRLSP KTNTCRGCNHLVCRDCRIQE SNGTWRCKVCSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	268
15N chemical shifts	61
1H chemical shifts	412

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RING domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, RING domain 76 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

LEU

GLU

2

ILE

LYS

ARG

LYS

GLY

ALA

LYS

ARG

GLY

SER

3

GLN

HIS

TYR

SER

ASP

ARG

THR

CYS

ALA

ARG

4

CYS

GLN

GLU

SER

LEU

GLY

ARG

LEU

SER

PRO

5

LYS

THR

ASN

THR

CYS

ARG

GLY

CYS

ASN

HIS

6

LEU

VAL

CYS

ARG

ASP

CYS

ARG

ILE

GLN

GLU

7

SER

ASN

GLY

THR

TRP

ARG

CYS

LYS

VAL

CYS

8

SER

GLY

PRO

SER

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RING domain, [U-13C; U-15N], 1.37 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; NTA 0.1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.925, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2CSZ
DBJ	BAC04287 BAF85277 BAG10233 BAG35911
EMBL	CAD97652 CAI42004 CAI42005
GB	AAH14913 AAQ14492 AIC57515 EAX02813 EAX02814
REF	NP_001123368 NP_001167539 NP_542775 XP_002808587 XP_003276489
SP	Q96C24
AlphaFold	Q96C24