BMRB Entry 11313

Name: Solution structure of the RING domain of the Zinc finger protein 183-like 1
Published: 2011-08-19

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PDB ID: 2csy
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11313
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the RING domain of the Zinc finger protein 183-like 1

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Sato, M.; Tomizawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Sato, M.; Tomizawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the RING domain of the Zinc finger protein 183-like 1" .

Assembly members:

Assembly members:
RING domain, polymer, 81 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P041206-06

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RING domain: GSSGSSGGSEEEEIPFRCFI CRQAFQNPVVTKCRHYFCES CALEHFRATPRCYICDQPTG GIFNPAKELMAKLQKSGPSS G

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	330
15N chemical shifts	72
1H chemical shifts	499

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RING domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, RING domain 81 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

SER

GLU

2

GLU

ILE

PRO

PHE

ARG

CYS

PHE

ILE

3

CYS

ARG

GLN

ALA

PHE

GLN

ASN

PRO

VAL

4

THR

LYS

CYS

ARG

HIS

TYR

PHE

CYS

GLU

SER

5

CYS

ALA

LEU

GLU

HIS

PHE

ARG

ALA

THR

PRO

6

ARG

CYS

TYR

ILE

CYS

ASP

GLN

PRO

THR

GLY

7

GLY

ILE

PHE

ASN

PRO

ALA

LYS

GLU

LEU

MET

8

ALA

LYS

LEU

GLN

LYS

SER

GLY

PRO

SER

9

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RING domain, [U-13C; U-15N], 1.47 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; EDTA 0.05 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.925, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2CSY
GB	EHH58672
REF	XP_001089736 XP_005586186 XP_011732534 XP_011732543