BMRB Entry 11312

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11312
MolProbity Validation Chart

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Title:
Solution structure of the C2H2 type zinc-binding domain of human zinc finger protein 64, isoforms 1 and 2
Deposition date:
2010-08-10
Original release date:
2011-08-19
Authors:
Yoneyama, M.; Koshiba, S.; Tochio, N.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Yoneyama, M.; Koshiba, S.; Tochio, N.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C2H2 type zinc-binding domain of human zinc finger protein 64, isoforms 1 and 2"  .

Assembly members:

Assembly members:
C2H2 type zinc-binding domain, polymer, 70 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P040809-09

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C2H2 type zinc-binding domain: GSSGSSGHPEKCSECSYSCS SKAALRIHERIHCTDRPFKC NYCSFDTKQPSNLSKHMKKF HGDMSGPSSG

Data sets:
Data typeCount
13C chemical shifts249
15N chemical shifts51
1H chemical shifts372

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1C2H2 type zinc-binding domain1
2ZINC ION no.12
3ZINC ION no.22

Entities:

Entity 1, C2H2 type zinc-binding domain 70 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYHISPROGLU
2   LYSCYSSERGLUCYSSERTYRSERCYSSER
3   SERLYSALAALALEUARGILEHISGLUARG
4   ILEHISCYSTHRASPARGPROPHELYSCYS
5   ASNTYRCYSSERPHEASPTHRLYSGLNPRO
6   SERASNLEUSERLYSHISMETLYSLYSPHE
7   HISGLYASPMETSERGLYPROSERSERGLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: C2H2 type zinc-binding domain, [U-13C; U-15N], 1.25 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 50 uM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.921, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

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