BMRB Entry 11309

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PDB ID: 1wjv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11309
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the N-terminal zinc finger domain of mouse cell growth regulating nucleolar protein LYAR

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Muto, Y.; Yokoyama, S.

Citation:

Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Muto, Y.; Yokoyama, S.. "Solution structure of the N-terminal zinc finger domain of mouse cell growth regulating nucleolar protein LYAR" .

Assembly members:

Assembly members:
Zinc finger DNA binding domain, polymer, 79 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P030421-25

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Zinc finger DNA binding domain: GSSGSSGMVFFTCNACGESV KKIQVEKHVSNCRNCECLSC IDCGKDFWGDDYKSHVKCIS EGQKYGGKGYEAKSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	299
15N chemical shifts	72
1H chemical shifts	452

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Zinc finger DNA binding domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, Zinc finger DNA binding domain 79 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

VAL

PHE

2

PHE

THR

CYS

ASN

ALA

CYS

GLY

GLU

SER

VAL

3

LYS

ILE

GLN

VAL

GLU

LYS

HIS

VAL

SER

4

ASN

CYS

ARG

ASN

CYS

GLU

CYS

LEU

SER

CYS

5

ILE

ASP

CYS

GLY

LYS

ASP

PHE

TRP

GLY

ASP

6

ASP

TYR

LYS

SER

HIS

VAL

LYS

CYS

ILE

SER

7

GLU

GLY

GLN

LYS

TYR

GLY

LYS

GLY

TYR

8

GLU

ALA

LYS

SER

GLY

PRO

SER

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: zinc finger DNA binding domain, [U-13C; U-15N], 1 mM; d-Tris-HCl 20 mM; NaCl 200 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 100 uM; H2O 90%; D2O 10%

condition_1: ionic strength: 220 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delagio, F. - processing

NMRView v5.0.4, Jhonson, B. A. - data analysis

Kujira v0.896, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WJV
DBJ	BAB24554 BAC36458
GB	AAB26644 AAI16924 AAI16928 EDL37545 EDL37546
REF	NP_079557 XP_006503822 XP_006503823 XP_006503824 XP_006503825
SP	Q08288
AlphaFold	Q08288