BMRB Entry 11307

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PDB ID: 1wim
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11307
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the RING finger Domain of the human UbcM4-interacting Protein 4

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the RING finger Domain of the human UbcM4-interacting Protein 4" .

Assembly members:

Assembly members:
RING finger Domain, polymer, 94 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P030609-14

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RING finger Domain: GSSGSSGCKLCLGEYPVEQM TTIAQCQCIFCTLCLKQYVE LLIKEGLETAISCPDAACPK QGHLQENEIECMVAAEIMQR YKKLQFERSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	391
15N chemical shifts	91
1H chemical shifts	630

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RING finger Domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, RING finger Domain 94 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

CYS

LYS

LEU

2

CYS

LEU

GLY

GLU

TYR

PRO

VAL

GLU

GLN

MET

3

THR

ILE

ALA

GLN

CYS

GLN

CYS

ILE

PHE

4

CYS

THR

LEU

CYS

LEU

LYS

GLN

TYR

VAL

GLU

5

LEU

ILE

LYS

GLU

GLY

LEU

GLU

THR

ALA

6

ILE

SER

CYS

PRO

ASP

ALA

CYS

PRO

LYS

7

GLN

GLY

HIS

LEU

GLN

GLU

ASN

GLU

ILE

GLU

8

CYS

MET

VAL

ALA

GLU

ILE

MET

GLN

ARG

9

TYR

LYS

LEU

GLN

PHE

GLU

ARG

SER

GLY

10

PRO

SER

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RING finger Domain, [U-13C; U-15N], 1.15 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.8996, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WIM
DBJ	BAA11478 BAC30058 BAD32182 BAE21715 BAG09632
GB	AAH30187 AAH50373 AAK51468 AAX82010 ABZ92280
REF	NP_001075446 NP_001075879 NP_055561 NP_542130 XP_001118400
SP	P50876 Q925F3
TPG	DAA24481
AlphaFold	P50876 Q925F3