BMRB Entry 11305

Name: Solution Structure of the LIM Domain of the Human Actin Binding LIM Protein 2
Published: 2011-08-19

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PDB ID: 1v6g
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11305
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the LIM Domain of the Human Actin Binding LIM Protein 2

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the LIM Domain of the Human Actin Binding LIM Protein 2" .

Assembly members:

Assembly members:
LIM Domain, polymer, 81 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P020930-36

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LIM Domain: GSSGSSGLDYQRLYGTRCFS CDQFIEGEVVSALGKTYHPD CFVCAVCRLPFPPGDRVTFN GKECMCQKCSLPVSVSGPSS G

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	308
15N chemical shifts	65
1H chemical shifts	463

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LIM Domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, LIM Domain 81 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

LEU

ASP

TYR

2

GLN

ARG

LEU

TYR

GLY

THR

ARG

CYS

PHE

SER

3

CYS

ASP

GLN

PHE

ILE

GLU

GLY

GLU

VAL

4

SER

ALA

LEU

GLY

LYS

THR

TYR

HIS

PRO

ASP

5

CYS

PHE

VAL

CYS

ALA

VAL

CYS

ARG

LEU

PRO

6

PHE

PRO

GLY

ASP

ARG

VAL

THR

PHE

ASN

7

GLY

LYS

GLU

CYS

MET

CYS

GLN

LYS

CYS

SER

8

LEU

PRO

VAL

SER

VAL

SER

GLY

PRO

SER

9

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: LIM Domain, [U-13C; U-15N], 1.22 mM; d-Tris-HCl 20 mM; NaCl 100 mM; NaN3 0.02%; ZnCl2 0.1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.863, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1V6G
REF	XP_004038463 XP_008577003 XP_009204733 XP_009204734 XP_009204735