BMRB Entry 11302

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PDB ID: 2dmd
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11302
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the N-terminal C2H2 type zinc-binding domain of the Zinc finger protein 64, isoforms 1 and 2

Deposition date:

2010-08-09

Original release date:

2011-08-19

Authors:

Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the N-terminal C2H2 type zinc-binding domain of the Zinc finger protein 64, isoforms 1 and 2" .

Assembly members:

Assembly members:
C2H2-type zinc-binding domain, polymer, 96 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050815-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C2H2-type zinc-binding domain: GSSGSSGPHKCEVCGKCFSR KDKLKTHMRCHTGVKPYKCK TCDYAAADSSSLNKHLRIHS DERPFKCQICPYASRNSSQL TVHLRSHTGDSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	373
15N chemical shifts	75
1H chemical shifts	561

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C2H2-type zinc-binding domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2
4	ZINC ION no.3	2

Entities:

Entity 1, C2H2-type zinc-binding domain 96 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

HIS

LYS

2

CYS

GLU

VAL

CYS

GLY

LYS

CYS

PHE

SER

ARG

3

LYS

ASP

LYS

LEU

LYS

THR

HIS

MET

ARG

CYS

4

HIS

THR

GLY

VAL

LYS

PRO

TYR

LYS

CYS

LYS

5

THR

CYS

ASP

TYR

ALA

ASP

SER

6

SER

LEU

ASN

LYS

HIS

LEU

ARG

ILE

HIS

SER

7

ASP

GLU

ARG

PRO

PHE

LYS

CYS

GLN

ILE

CYS

8

PRO

TYR

ALA

SER

ARG

ASN

SER

GLN

LEU

9

THR

VAL

HIS

LEU

ARG

SER

HIS

THR

GLY

ASP

10

SER

GLY

PRO

SER

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: C2H2 type zinc-binding domain, [U-13C; U-15N], 1.28 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 50 uM; IDA 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9736, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2DMD
GB	EDL06563
REF	XP_012374462