BMRB Entry 11300

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11300
MolProbity Validation Chart

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Title:
Solution structure of the Myb_DNA-binding domain of human Cell division cycle 5-like protein
Deposition date:
2010-08-09
Original release date:
2011-08-19
Authors:
Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Myb_DNA-binding domain of human Cell division cycle 5-like protein"  .

Assembly members:

Assembly members:
Myb_DNA-binding domain, polymer, 70 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050822-18

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Myb_DNA-binding domain: GSSGSSGKGGVWRNTEDEIL KAAVMKYGKNQWSRIASLLH RKSAKQCKARWYEWLDPSIK KTEWSGPSSG

Data sets:
Data typeCount
13C chemical shifts273
15N chemical shifts66
1H chemical shifts442

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Myb_DNA-binding domain1

Entities:

Entity 1, Myb_DNA-binding domain 70 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSGLYGLY
2   VALTRPARGASNTHRGLUASPGLUILELEU
3   LYSALAALAVALMETLYSTYRGLYLYSASN
4   GLNTRPSERARGILEALASERLEULEUHIS
5   ARGLYSSERALALYSGLNCYSLYSALAARG
6   TRPTYRGLUTRPLEUASPPROSERILELYS
7   LYSTHRGLUTRPSERGLYPROSERSERGLY

Samples:

sample_1: Myb_DNA-binding domain, [U-13C; U-15N], 1.19 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9736, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

PDB
DBJ BAA20885 BAA24862 BAD32228 BAE27510 BAE29661
EMBL CAG00328 CAL49404 CDQ56126 CDQ59735
GB AAB61210 AAD05365 AAF47383 AAH01568 AAH03893
REF NP_001008202 NP_001070010 NP_001096658 NP_001131045 NP_001163313
SP A7SD85 O08837 Q2KJC1 Q6A068 Q99459
TPG DAA16567
AlphaFold A7SD85 O08837 Q2KJC1 Q6A068 Q99459

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks