BMRB Entry 11294

Name: Solution structure of the RWD domain of human RWD omain containing protein 2
Published: 2011-08-19

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PDB ID: 2daw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11294
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the RWD domain of human RWD omain containing protein 2

Deposition date:

2010-08-09

Original release date:

2011-08-19

Authors:

Yoneyama, M.; Kigawa, T.; Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Yoneyama, M.; Kigawa, T.; Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the RWD domain of human RWD omain containing protein 2" .

Assembly members:

Assembly members:
RWD domain, polymer, 154 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050314-15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RWD domain: GSSGSSGMSASVKESLQLQL LEMEMLFSMFPNQGEVKLED VNALTNIKRYLEGTREALPP KIEFVITLQIEEPKVKIDLQ VTMPHSYPYLALQLFGRSSE LDRHQQLLLNKGLTSYIGTF DPGELCVCAAIQWLQDNSAS YFLNRKLVSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	634
15N chemical shifts	147
1H chemical shifts	1065

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RWD domain	1

Entities:

Entity 1, RWD domain 154 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

SER

ALA

2

SER

VAL

LYS

GLU

SER

LEU

GLN

LEU

GLN

LEU

3

LEU

GLU

MET

GLU

MET

LEU

PHE

SER

MET

PHE

4

PRO

ASN

GLN

GLY

GLU

VAL

LYS

LEU

GLU

ASP

5

VAL

ASN

ALA

LEU

THR

ASN

ILE

LYS

ARG

TYR

6

LEU

GLU

GLY

THR

ARG

GLU

ALA

LEU

PRO

7

LYS

ILE

GLU

PHE

VAL

ILE

THR

LEU

GLN

ILE

8

GLU

PRO

LYS

VAL

LYS

ILE

ASP

LEU

GLN

9

VAL

THR

MET

PRO

HIS

SER

TYR

PRO

TYR

LEU

10

ALA

LEU

GLN

LEU

PHE

GLY

ARG

SER

GLU

11

LEU

ASP

ARG

HIS

GLN

LEU

ASN

12

LYS

GLY

LEU

THR

SER

TYR

ILE

GLY

THR

PHE

13

ASP

PRO

GLY

GLU

LEU

CYS

VAL

CYS

ALA

14

ILE

GLN

TRP

LEU

GLN

ASP

ASN

SER

ALA

SER

15

TYR

PHE

LEU

ASN

ARG

LYS

LEU

VAL

SER

GLY

16

PRO

SER

GLY

Samples:

sample_1: RWD domain, [U-13C; U-15N], 1.17 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9318, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2DAW
GB	AAH10930 AAI04817 AAI12004 ABM83592 ABM87092
REF	NP_219479 XP_003816428 XP_004044388 XP_004044389 XP_011533706
SP	Q9UIY3
AlphaFold	Q9UIY3