BMRB Entry 11293

Name: Solution structure of the LIM domain of human Cysteine-rich protein 2
Published: 2011-08-19

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PDB ID: 2cu8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11293
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the LIM domain of human Cysteine-rich protein 2

Deposition date:

2010-08-09

Original release date:

2011-08-19

Authors:

Yoneyama, M.; Sasagawa, A.; Tomizawa, T.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Yoneyama, M.; Sasagawa, A.; Tomizawa, T.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the LIM domain of human Cysteine-rich protein 2" .

Assembly members:

Assembly members:
LIM domain, polymer, 76 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040614-03

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LIM domain: GSSGSSGMASKCPKCDKTVY FAEKVSSLGKDWHKFCLKCE RCSKTLTPGGHAEHDGKPFC HKPCYATLFGSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	300
15N chemical shifts	62
1H chemical shifts	443

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LIM domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, LIM domain 76 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

ALA

SER

2

LYS

CYS

PRO

LYS

CYS

ASP

LYS

THR

VAL

TYR

3

PHE

ALA

GLU

LYS

VAL

SER

LEU

GLY

LYS

4

ASP

TRP

HIS

LYS

PHE

CYS

LEU

LYS

CYS

GLU

5

ARG

CYS

SER

LYS

THR

LEU

THR

PRO

GLY

6

HIS

ALA

GLU

HIS

ASP

GLY

LYS

PRO

PHE

CYS

7

HIS

LYS

PRO

CYS

TYR

ALA

THR

LEU

PHE

GLY

8

SER

GLY

PRO

SER

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: LIM domain, [U-13C; U-15N], 0.44 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 2 mM; NaN3 0.02%; ZnCl2 0.01 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 600 MHz

PDB	2CU8
DBJ	BAH13206
GB	EDL18559 EDL97393 EDL97397 EFB24298 EGV94916
REF	NP_001257766 XP_002189362 XP_002808516 XP_002928938 XP_004055856