BMRB Entry 11292

Click here to enlarge.

PDB ID: 1x5z
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11292
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of the fibronectin type-III domain of human protein tyrosine phosphatase, receptor type, D isoform 4 variant

Deposition date:

2010-08-09

Original release date:

2011-08-18

Authors:

Yoneyama, M.; Saito, K.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Yoneyama, M.; Saito, K.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the fibronectin type-III domain of human protein tyrosine phosphatase, receptor type, D isoform 4 variant" .

Assembly members:

Assembly members:
Fibronectin type-III domain, polymer, 115 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050131-05

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Fibronectin type-III domain: GSSGSSGDIQVITQTGVPGQ PLNFKAEPESETSILLSWTP PRSDTIANYELVYKDGEHGE EQRITIEPGTSYRLQGLKPN SLYYFRLAARSPQGLGASTA EISARTMQSSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	443
15N chemical shifts	107
1H chemical shifts	715

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Fibronectin type-III domain	1

Entities:

Entity 1, Fibronectin type-III domain 115 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASP

ILE

GLN

2

VAL

ILE

THR

GLN

THR

GLY

VAL

PRO

GLY

GLN

3

PRO

LEU

ASN

PHE

LYS

ALA

GLU

PRO

GLU

SER

4

GLU

THR

SER

ILE

LEU

SER

TRP

THR

PRO

5

PRO

ARG

SER

ASP

THR

ILE

ALA

ASN

TYR

GLU

6

LEU

VAL

TYR

LYS

ASP

GLY

GLU

HIS

GLY

GLU

7

GLU

GLN

ARG

ILE

THR

ILE

GLU

PRO

GLY

THR

8

SER

TYR

ARG

LEU

GLN

GLY

LEU

LYS

PRO

ASN

9

SER

LEU

TYR

PHE

ARG

LEU

ALA

ARG

10

SER

PRO

GLN

GLY

LEU

GLY

ALA

SER

THR

ALA

11

GLU

ILE

SER

ALA

ARG

THR

MET

GLN

SER

12

GLY

PRO

SER

GLY

Samples:

sample_1: fn3 domain, [U-13C; U-15N], 1.37 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1X5Z
DBJ	BAD92106
GB	AAI06714 AAI06715 AAI06716 AAI06717 EAW58721
REF	NP_001035802 NP_001164496 NP_569075 NP_569076 NP_569077