BMRB Entry 11291

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PDB ID: 1x5y
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11291
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the fibronectin type-III domain of mouse myosin-binding protein C, Fast-type homolog

Deposition date:

2010-08-09

Original release date:

2011-08-18

Authors:

Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the fibronectin type-III domain of mouse myosin-binding protein C, Fast-type homolog" .

Assembly members:

Assembly members:
Fibronectin type-III domain, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P041012-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Fibronectin type-III domain: GSSGSSGPTSAPQHLTVEDV TDTTTTLKWRPPDRIGAGGI DGYLVEYCLEGSEEWVPANK EPVERCGFTVKDLPTGARIL FRVVGVNIAGRSEPATLLQP VTIRESGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	424
15N chemical shifts	97
1H chemical shifts	692

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Fibronectin type-III domain	1

Entities:

Entity 1, Fibronectin type-III domain 111 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

THR

SER

2

ALA

PRO

GLN

HIS

LEU

THR

VAL

GLU

ASP

VAL

3

THR

ASP

THR

LEU

LYS

TRP

ARG

4

PRO

ASP

ARG

ILE

GLY

ALA

GLY

ILE

5

ASP

GLY

TYR

LEU

VAL

GLU

TYR

CYS

LEU

GLU

6

GLY

SER

GLU

TRP

VAL

PRO

ALA

ASN

LYS

7

GLU

PRO

VAL

GLU

ARG

CYS

GLY

PHE

THR

VAL

8

LYS

ASP

LEU

PRO

THR

GLY

ALA

ARG

ILE

LEU

9

PHE

ARG

VAL

GLY

VAL

ASN

ILE

ALA

GLY

10

ARG

SER

GLU

PRO

ALA

THR

LEU

GLN

PRO

11

VAL

THR

ILE

ARG

GLU

SER

GLY

PRO

SER

12

GLY

Samples:

sample_1: fn3 domain, [U-13C; U-15N], 1.47 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 700 MHz