BMRB Entry 11289

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11289
MolProbity Validation Chart

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Title:
Solution structure of the LIM domain of human Leupaxin
Deposition date:
2010-08-09
Original release date:
2011-08-18
Authors:
Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the LIM domain of human Leupaxin"  .

Assembly members:

Assembly members:
LIM domain, polymer, 80 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P040809-04

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LIM domain: GSSGSSGKDFLAMFSPKCGG CNRPVLENYLSAMDTVWHPE CFVCGDCFTSFSTGSFFELD GRPFCELHYHHRRGSGPSSG

Data sets:
Data typeCount
13C chemical shifts322
15N chemical shifts72
1H chemical shifts463

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1LIM domain1
2ZINC ION no.12
3ZINC ION no.22

Entities:

Entity 1, LIM domain 80 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSASPPHE
2   LEUALAMETPHESERPROLYSCYSGLYGLY
3   CYSASNARGPROVALLEUGLUASNTYRLEU
4   SERALAMETASPTHRVALTRPHISPROGLU
5   CYSPHEVALCYSGLYASPCYSPHETHRSER
6   PHESERTHRGLYSERPHEPHEGLULEUASP
7   GLYARGPROPHECYSGLULEUHISTYRHIS
8   HISARGARGGLYSERGLYPROSERSERGLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: LIM domain, [U-13C; U-15N], 1.06 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

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