BMRB Entry 11288

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PDB ID: 1x3d
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11288
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the fibronectin type-III domain of human fibronectin type-III domain containing protein 3a

Deposition date:

2010-08-09

Original release date:

2011-08-18

Authors:

Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the fibronectin type-III domain of human fibronectin type-III domain containing protein 3a" .

Assembly members:

Assembly members:
Fibronectin type-III domain, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040628-01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Fibronectin type-III domain: GSSGSSGAEIFTTLSCEPDI PNPPRIANRTKNSLTLQWKA PSDNGSKIQNFVLEWDEGKG NGEFCQCYMGSQKQFKITKL SPAMGCKFRLSARNDYGTSG FSEEVLYYTSGCSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	472
15N chemical shifts	115
1H chemical shifts	728

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Fibronectin type-III domain	1

Entities:

Entity 1, Fibronectin type-III domain 118 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ALA

GLU

ILE

2

PHE

THR

LEU

SER

CYS

GLU

PRO

ASP

ILE

3

PRO

ASN

PRO

ARG

ILE

ALA

ASN

ARG

THR

4

LYS

ASN

SER

LEU

THR

LEU

GLN

TRP

LYS

ALA

5

PRO

SER

ASP

ASN

GLY

SER

LYS

ILE

GLN

ASN

6

PHE

VAL

LEU

GLU

TRP

ASP

GLU

GLY

LYS

GLY

7

ASN

GLY

GLU

PHE

CYS

GLN

CYS

TYR

MET

GLY

8

SER

GLN

LYS

GLN

PHE

LYS

ILE

THR

LYS

LEU

9

SER

PRO

ALA

MET

GLY

CYS

LYS

PHE

ARG

LEU

10

SER

ALA

ARG

ASN

ASP

TYR

GLY

THR

SER

GLY

11

PHE

SER

GLU

VAL

LEU

TYR

THR

SER

12

GLY

CYS

SER

GLY

PRO

SER

GLY

Samples:

sample_1: fn3 domain, [U-13C; U-15N], 1.16 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 5 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz