BMRB Entry 11287

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PDB ID: 1x3c
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11287
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the C2H2 type zinc-binding domain of human zinc finger protein 292

Deposition date:

2010-08-09

Original release date:

2011-08-18

Authors:

Yoneyama, M.; Tomizawa, T.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Yoneyama, M.; Tomizawa, T.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C2H2 type zinc-binding domain of human zinc finger protein 292" .

Assembly members:

Assembly members:
C2H2 type zinc-binding domain, polymer, 73 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P041018-02

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C2H2 type zinc-binding domain: GSSGSSGRKKPVSQSLEFPT RYSPYRPYRCVHQGCFAAFT IQQNLILHYQAVHKSDLPAF SAEVEEESGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	297
15N chemical shifts	62
1H chemical shifts	450

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C2H2 type zinc-binding domain	1
2	ZINC ION	2

Entities:

Entity 1, C2H2 type zinc-binding domain 73 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ARG

LYS

2

PRO

VAL

SER

GLN

SER

LEU

GLU

PHE

PRO

THR

3

ARG

TYR

SER

PRO

TYR

ARG

PRO

TYR

ARG

CYS

4

VAL

HIS

GLN

GLY

CYS

PHE

ALA

PHE

THR

5

ILE

GLN

ASN

LEU

ILE

LEU

HIS

TYR

GLN

6

ALA

VAL

HIS

LYS

SER

ASP

LEU

PRO

ALA

PHE

7

SER

ALA

GLU

VAL

GLU

SER

GLY

PRO

8

SER

GLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: C2H2 type zinc-binding domain, [U-13C; U-15N], 1.90 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; NTA 0.1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1X3C
DBJ	BAA25456 BAB14654 BAI45414
EMBL	CAD97823
GB	AAB06954 AAH63594 AAI72360 AAV86070 AEB61065
REF	NP_001179779 NP_055836 XP_001088554 XP_002806763 XP_002817165
SP	O60281
TPG	DAA26182
AlphaFold	O60281