BMRB Entry 11282

Name: Solution Structure of the UBA domain in Human Protein FAM100B
Published: 2011-08-18

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PDB ID: 2dzl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11282
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the UBA domain in Human Protein FAM100B

Deposition date:

2010-08-09

Original release date:

2011-08-18

Authors:

Zhao, C.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Zhao, C.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the UBA domain in Human Protein FAM100B" .

Assembly members:

Assembly members:
UBA-like domain, residues 8-66, polymer, 66 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060417-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UBA-like domain, residues 8-66: GSSGSSGMSVNMDELRHQVM INQFVLAAGCAADQAKQLLQ AAHWQFETALSTFFQETNIP NSHHHH

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	234
15N chemical shifts	63
1H chemical shifts	373

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UBA-like domain, residues 8-66	1

Entities:

Entity 1, UBA-like domain, residues 8-66 66 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

SER

VAL

2

ASN

MET

ASP

GLU

LEU

ARG

HIS

GLN

VAL

MET

3

ILE

ASN

GLN

PHE

VAL

LEU

ALA

GLY

CYS

4

ALA

ASP

GLN

ALA

LYS

GLN

LEU

GLN

5

ALA

HIS

TRP

GLN

PHE

GLU

THR

ALA

LEU

6

SER

THR

PHE

GLN

GLU

THR

ASN

ILE

PRO

7

ASN

SER

HIS

Samples:

sample_1: UBA-like domain, [U-13C; U-15N], 0.43 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296.0 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9748, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2DZL
GB	AAH35511 AAH60859 ABW03765 EAW89383 EHA99413
REF	NP_872371 XP_001151089 XP_002696214 XP_003340352 XP_003358005
SP	Q8IYN6
TPG	DAA18129
AlphaFold	Q8IYN6