BMRB Entry 11278

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PDB ID: 2di0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11278
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the CUE Domain in the Human Activating Signal Cointegrator 1 Complex Subunit 2 (ASCC2)

Deposition date:

2010-08-09

Original release date:

2011-08-18

Authors:

Zhao, C.; Kigawa, T.; Tochio, N.; Koshiba, S.; Harada, T.; Watanabe, S.; Yokoyama, S.

Citation:

Citation: Zhao, C.; Kigawa, T.; Tochio, N.; Koshiba, S.; Harada, T.; Watanabe, S.; Yokoyama, S.. "Solution Structure of the CUE Domain in the Human Activating Signal Cointegrator 1 Complex Subunit 2 (ASCC2)" .

Assembly members:

Assembly members:
CUE domain, polymer, 71 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060116-03

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CUE domain: GSSGSSGMCGVELDSLISQV KDLLPDLGEGFILACLEYYH YDPEQVINNILEERLAPTLS QLDRNLDREMN

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	296
15N chemical shifts	70
1H chemical shifts	482

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CUE domain	1

Entities:

Entity 1, CUE domain 71 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

CYS

GLY

2

VAL

GLU

LEU

ASP

SER

LEU

ILE

SER

GLN

VAL

3

LYS

ASP

LEU

PRO

ASP

LEU

GLY

GLU

GLY

4

PHE

ILE

LEU

ALA

CYS

LEU

GLU

TYR

HIS

5

TYR

ASP

PRO

GLU

GLN

VAL

ILE

ASN

ILE

6

LEU

GLU

ARG

LEU

ALA

PRO

THR

LEU

SER

7

GLN

LEU

ASP

ARG

ASN

LEU

ASP

ARG

GLU

MET

8

ASN

Samples:

sample_1: CUE domain, [U-13C; U-15N], 1.24 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296.0 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9732, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 900 MHz