BMRB Entry 11277

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11277
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution Structure of the RA Domain in the Human Link Guanine Nucleotide Exchange Factor II (Link-GEFII)
Deposition date:
2010-08-09
Original release date:
2011-08-18
Authors:
Zhao, C.; Kigawa, T.; Yoneyama, M.; Koshiba, S.; Harada, T.; Watanabe, S.; Yokoyama, S.
Citation:

Citation: Zhao, C.; Kigawa, T.; Yoneyama, M.; Koshiba, S.; Harada, T.; Watanabe, S.; Yokoyama, S.. "Solution Structure of the RA Domain in the Human Link Guanine Nucleotide Exchange Factor II (Link-GEFII)"  .

Assembly members:

Assembly members:
RA domain, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P051212-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RA domain: GSSGSSGDEIFCRVYMPDHS YVTIRSRLSASVQDILGSVT EKLQYSEEPAGREDSLILVA VSSSGEKVLLQPTEDCVFTA LGINSHLFACTRDSYEALVP LPEEIQVSPGDTEISGPSSG

Data sets:
Data typeCount
13C chemical shifts487
15N chemical shifts108
1H chemical shifts752

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RA domain1

Entities:

Entity 1, RA domain 120 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASPGLUILE
2   PHECYSARGVALTYRMETPROASPHISSER
3   TYRVALTHRILEARGSERARGLEUSERALA
4   SERVALGLNASPILELEUGLYSERVALTHR
5   GLULYSLEUGLNTYRSERGLUGLUPROALA
6   GLYARGGLUASPSERLEUILELEUVALALA
7   VALSERSERSERGLYGLULYSVALLEULEU
8   GLNPROTHRGLUASPCYSVALPHETHRALA
9   LEUGLYILEASNSERHISLEUPHEALACYS
10   THRARGASPSERTYRGLUALALEUVALPRO
11   LEUPROGLUGLUILEGLNVALSERPROGLY
12   ASPTHRGLUILESERGLYPROSERSERGLY

Samples:

sample_1: RA domain, [U-13C; U-15N], 1.21 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296.0 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9732, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAG36274 BAG57820 BAH14121
EMBL CAH91648
GB AAF22003 AAI15375 AAI15376 ABG81429 EAW60645
REF NP_001125968 NP_001192336 NP_001290462 NP_001290463 NP_057423
SP Q5R9B2 Q9UHV5
AlphaFold Q5R9B2 Q9UHV5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks