BMRB Entry 11275

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PDB ID: 2dhx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11275
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the RRM Domain in the Human Poly (ADP-ribose) Polymerase Family, Member 10 Variant

Deposition date:

2010-08-09

Original release date:

2011-08-18

Authors:

Zhao, C.; Kigawa, T.; Tochio, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Zhao, C.; Kigawa, T.; Tochio, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution Structure of the RRM Domain in the Human Poly (ADP-ribose) Polymerase Family, Member 10 Variant" .

Assembly members:

Assembly members:
RRM domain, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050822-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RRM domain: GSSGSSGGVAVEVRGLPPAV PDELLTLYFENRRRSGGGPV LSWQRLGCGGVLTFREPADA ERVLAQADHELHGAQLSLRP APPRAPARLLLQGLPPGTSG PSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	411
15N chemical shifts	92
1H chemical shifts	625

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RRM domain	1

Entities:

Entity 1, RRM domain 104 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

VAL

ALA

2

VAL

GLU

VAL

ARG

GLY

LEU

PRO

ALA

VAL

3

PRO

ASP

GLU

LEU

THR

LEU

TYR

PHE

GLU

4

ASN

ARG

SER

GLY

PRO

VAL

5

LEU

SER

TRP

GLN

ARG

LEU

GLY

CYS

GLY

6

VAL

LEU

THR

PHE

ARG

GLU

PRO

ALA

ASP

ALA

7

GLU

ARG

VAL

LEU

ALA

GLN

ALA

ASP

HIS

GLU

8

LEU

HIS

GLY

ALA

GLN

LEU

SER

LEU

ARG

PRO

9

ALA

PRO

ARG

ALA

PRO

ALA

ARG

LEU

10

LEU

GLN

GLY

LEU

PRO

GLY

THR

SER

GLY

11

PRO

SER

GLY

Samples:

sample_1: RRM domain, [U-13C; U-15N], 1.22 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296.0 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9732, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz