BMRB Entry 11272

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PDB ID: 2daj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11272
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the Novel Identified Ubiquitin-like Domain in the Human COBL-like 1 Protein

Deposition date:

2010-08-09

Original release date:

2011-08-18

Authors:

Zhao, C.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Zhao, C.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution Structure of the Novel Identified Ubiquitin-like Domain in the Human COBL-like 1 Protein" .

Assembly members:

Assembly members:
ubiquitin-like domain, polymer, 91 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050719-08

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ubiquitin-like domain: GSSGSSGEKTVRVVINFKKT QKTIVRVSPHASLQELAPII CSKCEFDPLHTLLLKDYQSQ EPLDLTKSLNDLGLRELYAM DVNRESGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	384
15N chemical shifts	83
1H chemical shifts	613

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ubiquitin-like domain	1

Entities:

Entity 1, ubiquitin-like domain 91 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLU

LYS

THR

2

VAL

ARG

VAL

ILE

ASN

PHE

LYS

THR

3

GLN

LYS

THR

ILE

VAL

ARG

VAL

SER

PRO

HIS

4

ALA

SER

LEU

GLN

GLU

LEU

ALA

PRO

ILE

5

CYS

SER

LYS

CYS

GLU

PHE

ASP

PRO

LEU

HIS

6

THR

LEU

LYS

ASP

TYR

GLN

SER

GLN

7

GLU

PRO

LEU

ASP

LEU

THR

LYS

SER

LEU

ASN

8

ASP

LEU

GLY

LEU

ARG

GLU

LEU

TYR

ALA

MET

9

ASP

VAL

ASN

ARG

GLU

SER

GLY

PRO

SER

10

GLY

Samples:

sample_1: ubiquitin-like domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298.0 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9321, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2DAJ
DBJ	BAA76821 BAG09927 BAH11931
EMBL	CAH89676
GB	AAH71588 AAX93068 EAX11339 EAX11340 EFB27997
REF	NP_001265387 NP_001265389 NP_001265390 NP_055715 XP_001098489
SP	Q53SF7
AlphaFold	Q53SF7