BMRB Entry 11271

Name: Solution Structure of the C-terminal UBA Domain in the Human Ubiquilin 3
Published: 2011-08-18

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PDB ID: 2dah
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11271
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the C-terminal UBA Domain in the Human Ubiquilin 3

Deposition date:

2010-08-09

Original release date:

2011-08-18

Authors:

Zhao, C.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Zhao, C.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution Structure of the C-terminal UBA Domain in the Human Ubiquilin 3" .

Assembly members:

Assembly members:
UBA domain, polymer, 54 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050613-28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UBA domain: GSSGSSGHFQVQLEQLRSMG FLNREANLQALIATGGDVDA AVEKLRQSSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	195
15N chemical shifts	52
1H chemical shifts	315

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UBA domain	1

Entities:

Entity 1, UBA domain 54 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

HIS

PHE

GLN

2

VAL

GLN

LEU

GLU

GLN

LEU

ARG

SER

MET

GLY

3

PHE

LEU

ASN

ARG

GLU

ALA

ASN

LEU

GLN

ALA

4

LEU

ILE

ALA

THR

GLY

ASP

VAL

ASP

ALA

5

ALA

VAL

GLU

LYS

LEU

ARG

GLN

SER

GLY

6

PRO

SER

GLY

Samples:

sample_1: UBA domain, [U-13C; U-15N], 0.80 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296.0 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9321, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 700 MHz

PDB	2DAH
DBJ	BAK62003 BAK63613
GB	AAF67143 AAG41675 AAH36743 ABW03695 EAW68790
REF	NP_059509 XP_002822162 XP_003254882 XP_003254883 XP_003312933
SP	Q9H347
AlphaFold	Q9H347