BMRB Entry 11263

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PDB ID: 1wx9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11263
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the N-terminal Ubiquitin-like Domain in the Human BAT3 Protein

Deposition date:

2010-08-09

Original release date:

2011-08-18

Authors:

Zhao, C.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Zhao, C.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the N-terminal Ubiquitin-like Domain in the Human BAT3 Protein" .

Assembly members:

Assembly members:
ubiquitin-like domain, polymer, 86 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040921-06

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ubiquitin-like domain: GSSGSSGLEVLVKTLDSQTR TFIVGAQMNVKEFKEHIAAS VSIPSEKQRLIYQGRVLQDD KKLQEYNVGGKVIHLVERAP SGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	350
15N chemical shifts	82
1H chemical shifts	566

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ubiquitin-like domain	1

Entities:

Entity 1, ubiquitin-like domain 86 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

LEU

GLU

VAL

2

LEU

VAL

LYS

THR

LEU

ASP

SER

GLN

THR

ARG

3

THR

PHE

ILE

VAL

GLY

ALA

GLN

MET

ASN

VAL

4

LYS

GLU

PHE

LYS

GLU

HIS

ILE

ALA

SER

5

VAL

SER

ILE

PRO

SER

GLU

LYS

GLN

ARG

LEU

6

ILE

TYR

GLN

GLY

ARG

VAL

LEU

GLN

ASP

7

LYS

LEU

GLN

GLU

TYR

ASN

VAL

GLY

8

LYS

VAL

ILE

HIS

LEU

VAL

GLU

ARG

ALA

PRO

9

SER

GLY

PRO

SER

GLY

Samples:

sample_1: ubiquitin-like domain, [U-13C; U-15N], 1.23 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298.0 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.921, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WX9 4EEW
EMBL	CAN59668
GB	ELW61850 EPY85374
REF	XP_003422254 XP_004390798 XP_004390799 XP_004390800 XP_004390801