BMRB Entry 11251

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11251
MolProbity Validation Chart

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Title:
The solution structure of the C-terminal region of Zinc finger FYVE domain-containing protein 21
Deposition date:
2010-08-03
Original release date:
2011-08-03
Authors:
Koshiba, Seizo; Tomizawa, Tadashi; Hayashi, Fumiaki; Tochio, Naoya; Harada, Takushi; Watanabe, Satoru; Kigawa, Takanori; Yokoyama, Shigeyuki
Citation:

Citation: Koshiba, Seizo; Tomizawa, Tadashi; Hayashi, Fumiaki; Tochio, Naoya; Harada, Takushi; Watanabe, Satoru; Kigawa, Takanori; Yokoyama, Shigeyuki. "The solution structure of the C-terminal region of Zinc finger FYVE domain-containing protein 21"  .

Assembly members:

Assembly members:
C-terminal region of ZFYVE21, polymer, 141 residues, 15233.231 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: E. coli - cell free   Vector: P070129-04

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-terminal region of ZFYVE21: GSSGSSGAEFYDKQLKVLLS GATFLVTFGNSEKPETMTCR LSNNQRYLFLDGDSHYEIEI VHISTVQILTEGFPPGGGNA RATGMFLQYTVPGTEGVTQL KLTVVEDVTVGRRQAVAWLV AMHKAAKLLYESRDQSGPSS G

Data sets:
Data typeCount
13C chemical shifts593
15N chemical shifts137
1H chemical shifts936

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal region of ZFYVE211

Entities:

Entity 1, C-terminal region of ZFYVE21 141 residues - 15233.231 Da.

1   GLYSERSERGLYSERSERGLYALAGLUPHE
2   TYRASPLYSGLNLEULYSVALLEULEUSER
3   GLYALATHRPHELEUVALTHRPHEGLYASN
4   SERGLULYSPROGLUTHRMETTHRCYSARG
5   LEUSERASNASNGLNARGTYRLEUPHELEU
6   ASPGLYASPSERHISTYRGLUILEGLUILE
7   VALHISILESERTHRVALGLNILELEUTHR
8   GLUGLYPHEPROPROGLYGLYGLYASNALA
9   ARGALATHRGLYMETPHELEUGLNTYRTHR
10   VALPROGLYTHRGLUGLYVALTHRGLNLEU
11   LYSLEUTHRVALVALGLUASPVALTHRVAL
12   GLYARGARGGLNALAVALALATRPLEUVAL
13   ALAMETHISLYSALAALALYSLEULEUTYR
14   GLUSERARGASPGLNSERGLYPROSERSER
15   GLY

Samples:

sample_1: C-terminal region of ZFYVE21, [U-13C; U-15N], 1.18 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 120 mM; pH: 7; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

Kujira, N. Kobayashi - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

PDB
DBJ BAF83208
EMBL CAD62589
GB AAH01130 AAH05999 AAR14313 AIC52221 EAW81844
REF NP_076976 XP_004055804 XP_008957301
SP Q9BQ24
AlphaFold Q9BQ24

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SPARKY: Backbone or all simulated peaks