BMRB Entry 11247

Name: Solution structure of the FHA domain of human ubiquitin ligase protein RNF8
Published: 2011-08-03

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PDB ID: 2csw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11247
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the FHA domain of human ubiquitin ligase protein RNF8

Deposition date:

2010-07-23

Original release date:

2011-08-03

Authors:

Hayashi, F.; Nagashima, T.; Yokoyama, S.

Citation:

Citation: Hayashi, F.; Nagashima, T.; Yokoyama, S.. "Solution structure of the FHA domain of human ubiquitin ligase protein RNF8" .

Assembly members:

Assembly members:
FHA domain, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040322-86

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FHA domain: GSSGSSGVTGDRAGGRSWCL RRVGMSAGWLLLEDGCEVTV GRGFGVTYQLVSKICPLMIS RNHCVLKQNPEGQWTIMDNK SLNGVWLNRARLEPLRVYSI HQGDYIQLGVPLENKENAEY EYEVTEEDWETIYPCLSPKS GPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	608
15N chemical shifts	147
1H chemical shifts	973

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FHA domain	1

Entities:

Entity 1, FHA domain 145 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

VAL

THR

GLY

2

ASP

ARG

ALA

GLY

ARG

SER

TRP

CYS

LEU

3

ARG

VAL

GLY

MET

SER

ALA

GLY

TRP

LEU

4

LEU

GLU

ASP

GLY

CYS

GLU

VAL

THR

VAL

5

GLY

ARG

GLY

PHE

GLY

VAL

THR

TYR

GLN

LEU

6

VAL

SER

LYS

ILE

CYS

PRO

LEU

MET

ILE

SER

7

ARG

ASN

HIS

CYS

VAL

LEU

LYS

GLN

ASN

PRO

8

GLU

GLY

GLN

TRP

THR

ILE

MET

ASP

ASN

LYS

9

SER

LEU

ASN

GLY

VAL

TRP

LEU

ASN

ARG

ALA

10

ARG

LEU

GLU

PRO

LEU

ARG

VAL

TYR

SER

ILE

11

HIS

GLN

GLY

ASP

TYR

ILE

GLN

LEU

GLY

VAL

12

PRO

LEU

GLU

ASN

LYS

GLU

ASN

ALA

GLU

TYR

13

GLU

TYR

GLU

VAL

THR

GLU

ASP

TRP

GLU

14

THR

ILE

TYR

PRO

CYS

LEU

SER

PRO

LYS

SER

15

GLY

PRO

SER

GLY

Samples:

sample_1: FHA domain, [U-13C; U-15N], 1.1 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9297, Kobayashi, N. - data analysis

CYANA v2.0, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz
Varian INOVA 900 MHz

PDB	2CSW 2PIE
DBJ	BAA31621 BAA33557 BAD96485 BAE87261 BAG09793
EMBL	CAH93334
GB	AAH07517 AAP36114 AAP36421 AAQ14887 AAX29228
REF	NP_001126963 NP_001252758 NP_001267209 NP_001274572 NP_003949
SP	O76064 Q5R4I2
AlphaFold	O76064 Q5R4I2